Detailed information of TA system TA127092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1719..2193
• Replicon: plasmid pSH12_5
• Accession: NZ_CP040838
• Organism: Klebsiella pneumoniae strain SH12

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: FHY43_RS29460
• Protein ID: WP_004197762.1
• Coordinates: 1719..1985 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FHY43_RS29465
• Protein ID: WP_000879771.1
• Coordinates: 1969..2193 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY43_RS29445 (FHY43_30055)	336..662	+	327	WP_050516389.1	hypothetical protein	-
FHY43_RS29450 (FHY43_30060)	688..1233	+	546	WP_023317533.1	hypothetical protein	-
FHY43_RS29455 (FHY43_30065)	1299..1649	+	351	WP_004197756.1	hypothetical protein	-
FHY43_RS29460 (FHY43_30070)	1719..1985	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FHY43_RS29465 (FHY43_30075)	1969..2193	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
FHY43_RS29930	2356..2484	+	129	WP_000523570.1	hypothetical protein	-
FHY43_RS29470 (FHY43_30080)	2860..3072	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
FHY43_RS29475 (FHY43_30085)	3079..3588	-	510	WP_032433972.1	MbeB family mobilization protein	-
FHY43_RS29935	3623..4057	-	435	Protein_8	relaxase/mobilization nuclease domain-containing protein	-
FHY43_RS29485 (FHY43_30095)	4266..4610	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
FHY43_RS29490	4600..4776	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T127092 WP_004197762.1 NZ_CP040838:c1985-1719 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T127092 NZ_CP040838:c1985-1719 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT127092 WP_000879771.1 NZ_CP040838:c2193-1969 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT127092 NZ_CP040838:c2193-1969 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5