Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2726833..2727449 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag FF956_RS12720 Protein ID WP_003407593.1
Coordinates 2726833..2727150 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag FF956_RS12725 Protein ID WP_003900349.1
Coordinates 2727147..2727449 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS12690 (FF956_13235) 2721842..2722870 - 1029 WP_003903478.1 glycosyltransferase family 2 protein -
FF956_RS12695 (FF956_13240) 2722915..2723313 - 399 WP_003900351.1 hypothetical protein -
FF956_RS12700 (FF956_13245) 2723374..2723586 + 213 WP_003898900.1 dodecin family protein -
FF956_RS12705 (FF956_13250) 2723707..2724417 + 711 WP_235639255.1 class I SAM-dependent methyltransferase -
FF956_RS12710 (FF956_13255) 2724490..2725779 - 1290 WP_003407599.1 serine hydrolase domain-containing protein -
FF956_RS12715 (FF956_13260) 2725832..2726836 - 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
FF956_RS12720 (FF956_13265) 2726833..2727150 - 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
FF956_RS12725 (FF956_13270) 2727147..2727449 - 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
FF956_RS12730 (FF956_13275) 2727463..2729715 - 2253 WP_003407587.1 methylmalonyl-CoA mutase -
FF956_RS12735 (FF956_13280) 2729716..2731563 - 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T126771 WP_003407593.1 NZ_CP040692:c2727150-2726833 [Mycobacterium tuberculosis complex sp. N0157]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T126771 NZ_CP040692:c2727150-2726833 [Mycobacterium tuberculosis complex sp. N0157]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT126771 WP_003900349.1 NZ_CP040692:c2727449-2727147 [Mycobacterium tuberculosis complex sp. N0157]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT126771 NZ_CP040692:c2727449-2727147 [Mycobacterium tuberculosis complex sp. N0157]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References