Detailed information of TA system TA126502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 636194..636870
• Replicon: chromosome
• Accession: NZ_CP040688
• Organism: Mycobacterium tuberculosis complex sp. N0052

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FF951_RS02860
• Protein ID: WP_003898500.1
• Coordinates: 636194..636607 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FF951_RS02865
• Protein ID: WP_003402915.1
• Coordinates: 636604..636870 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF951_RS02830 (FF951_02870)	631539..631841	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FF951_RS02835 (FF951_02875)	631901..632179	-	279	WP_003402901.1	hypothetical protein	-
FF951_RS02840 (FF951_02880)	632176..633429	-	1254	WP_003402904.1	inorganic phosphate transporter	-
FF951_RS02845 (FF951_02885)	633549..633935	-	387	WP_003402907.1	VOC family protein	-
FF951_RS02850 (FF951_02890)	633998..634882	-	885	WP_003402909.1	SDR family oxidoreductase	-
FF951_RS02855 (FF951_02895)	634978..635880	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FF951_RS02860 (FF951_02900)	636194..636607	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF951_RS02865 (FF951_02905)	636604..636870	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FF951_RS02870 (FF951_02910)	637062..638777	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FF951_RS02875 (FF951_02915)	638855..640459	+	1605	WP_003402920.1	amidohydrolase	-
FF951_RS02880 (FF951_02920)	640456..641436	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T126502 WP_003898500.1 NZ_CP040688:c636607-636194 [Mycobacterium tuberculosis complex sp. N0052]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T126502 NZ_CP040688:c636607-636194 [Mycobacterium tuberculosis complex sp. N0052]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT126502 WP_003402915.1 NZ_CP040688:c636870-636604 [Mycobacterium tuberculosis complex sp. N0052]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT126502 NZ_CP040688:c636870-636604 [Mycobacterium tuberculosis complex sp. N0052]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0