Detailed information of TA system TA126008

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53338..53602
• Replicon: plasmid pCR-HvKP5-p3
• Accession: NZ_CP040548
• Organism: Klebsiella pneumoniae strain CR-HvKP5

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: FFT49_RS29570
• Protein ID: WP_001303307.1
• Coordinates: 53450..53602 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	53338..53400 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT49_RS29555	49440..50510	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
FFT49_RS29560	50529..51737	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	51917..51977	-	61	NuclAT_1	-	-
-	51917..51977	-	61	NuclAT_1	-	-
-	51917..51977	-	61	NuclAT_1	-	-
-	51917..51977	-	61	NuclAT_1	-	-
FFT49_RS29565	52044..53135	-	1092	WP_000426061.1	hypothetical protein	-
-	53338..53400	-	63	NuclAT_0	-	Antitoxin
-	53338..53400	-	63	NuclAT_0	-	Antitoxin
-	53338..53400	-	63	NuclAT_0	-	Antitoxin
-	53338..53400	-	63	NuclAT_0	-	Antitoxin
FFT49_RS29570	53450..53602	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
FFT49_RS29575	53674..53925	-	252	WP_001291968.1	hypothetical protein	-
-	54312..54363	-	52	NuclAT_2	-	-
-	54312..54363	-	52	NuclAT_2	-	-
-	54312..54363	-	52	NuclAT_2	-	-
-	54312..54363	-	52	NuclAT_2	-	-
FFT49_RS30655	54849..55025	-	177	WP_001054900.1	hypothetical protein	-
FFT49_RS29590	55234..55443	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
FFT49_RS29595	55541..56155	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
FFT49_RS29600	56231..58399	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..99717	99717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T126008 WP_001303307.1 NZ_CP040548:53450-53602 [Klebsiella pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T126008 NZ_CP040548:53450-53602 [Klebsiella pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT126008 NZ_CP040548:c53400-53338 [Klebsiella pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin