Detailed information of TA system TA125972

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 176489..176742
• Replicon: plasmid pCR-HvKP4-pKPC
• Accession: NZ_CP040541
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: FFT48_RS29115
• Protein ID: WP_001312851.1
• Coordinates: 176593..176742 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	176489..176548 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS29070	172149..172214	-	66	Protein_221	helix-turn-helix domain-containing protein	-
FFT48_RS29075	172267..172971	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FFT48_RS29080	173035..173196	+	162	Protein_223	DNA helicase	-
FFT48_RS29085	173216..173962	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
FFT48_RS29090	174017..174577	+	561	WP_000139328.1	fertility inhibition protein FinO	-
FFT48_RS29095	174709..174909	+	201	WP_015059022.1	hypothetical protein	-
FFT48_RS29100	175295..175894	+	600	WP_032083981.1	hypothetical protein	-
FFT48_RS29105	176058..176288	+	231	WP_001736714.1	hypothetical protein	-
-	176489..176548	-	60	NuclAT_1	-	Antitoxin
-	176489..176548	-	60	NuclAT_1	-	Antitoxin
-	176489..176548	-	60	NuclAT_1	-	Antitoxin
-	176489..176548	-	60	NuclAT_1	-	Antitoxin
FFT48_RS29115	176593..176742	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
FFT48_RS29120	177026..177274	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..177585	177585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T125972 WP_001312851.1 NZ_CP040541:176593-176742 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T125972 NZ_CP040541:176593-176742 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT125972 NZ_CP040541:c176548-176489 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin