Detailed information of TA system TA125969

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37144..37413
• Replicon: plasmid pCR-HvKP4-pKPC
• Accession: NZ_CP040541
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FFT48_RS28100
• Protein ID: WP_001312861.1
• Coordinates: 37255..37413 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37144..37209 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS28075	32854..33381	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FFT48_RS28080	33439..33672	+	234	WP_000006003.1	DUF905 family protein	-
FFT48_RS28085	33733..35756	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
FFT48_RS28090	35825..36259	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FFT48_RS28095	36256..36975	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	37144..37209	+	66	-	-	Antitoxin
FFT48_RS28100	37255..37413	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FFT48_RS30575	37651..38028	-	378	Protein_49	hypothetical protein	-
FFT48_RS28120	38328..38624	+	297	WP_001272251.1	hypothetical protein	-
FFT48_RS28125	38735..39556	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FFT48_RS28130	39853..40500	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FFT48_RS28135	40777..41160	+	384	WP_000124981.1	relaxosome protein TraM	-
FFT48_RS28140	41351..42037	+	687	WP_015059009.1	PAS domain-containing protein	-
FFT48_RS28145	42131..42358	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..177585	177585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T125969 WP_001312861.1 NZ_CP040541:37255-37413 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T125969 NZ_CP040541:37255-37413 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT125969 NZ_CP040541:37144-37209 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin