Detailed information of TA system TA125875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 174499..175118
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2W1KCH5
• Locus tag: FE661_RS00845
• Protein ID: WP_031575563.1
• Coordinates: 174822..175118 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FE661_RS00840
• Protein ID: WP_031575560.1
• Coordinates: 174499..174816 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS00815	170029..170259	+	231	Protein_161	hypothetical protein	-
FE661_RS00820	171256..171996	+	741	WP_054608922.1	P-type conjugative transfer protein TrbJ	-
FE661_RS00825	172008..172262	+	255	WP_012535920.1	EexN family lipoprotein	-
FE661_RS00830	172276..173970	+	1695	WP_054608923.1	P-type conjugative transfer protein TrbL	-
FE661_RS00835	174041..174445	+	405	WP_031575557.1	hypothetical protein	-
FE661_RS00840	174499..174816	-	318	WP_031575560.1	putative addiction module antidote protein	Antitoxin
FE661_RS00845	174822..175118	-	297	WP_031575563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE661_RS00850	175339..175743	+	405	WP_031575566.1	hypothetical protein	-
FE661_RS00855	175736..176656	-	921	WP_146235851.1	hypothetical protein	-
FE661_RS00860	176806..177474	+	669	WP_054608924.1	type IV secretion system protein	-
FE661_RS00865	177487..178587	+	1101	WP_054608925.1	TrbG/VirB9 family P-type conjugative transfer protein	-
FE661_RS00870	178589..179593	+	1005	WP_054608926.1	TrbG/VirB9 family P-type conjugative transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11112.88 Da
• Isoelectric Point: 9.6531

>T125875 WP_031575563.1 NZ_CP040511:c175118-174822 [Acidithiobacillus ferrooxidans]
MLELIETNVFREWLEGLRDHQARARIQVRMRRLSLGNPGDVKPVGEGISEMRIDYGPGYRVYFMRRGPVVVALLCGGDKG
SQERDIALAKAIAAQWKE

Nucleotide

• Length: 297 bp

>T125875 NZ_CP040511:c175118-174822 [Acidithiobacillus ferrooxidans]
ATGCTTGAGCTCATAGAAACCAACGTGTTCAGGGAATGGCTCGAAGGTCTGCGCGACCACCAAGCCCGCGCAAGGATTCA
GGTGCGGATGCGCCGATTGAGCCTTGGGAACCCTGGAGACGTGAAGCCTGTAGGTGAAGGCATCTCGGAAATGCGGATTG
ACTACGGTCCTGGCTATCGCGTCTATTTCATGAGGCGCGGACCAGTTGTGGTGGCGCTTCTGTGCGGCGGAGACAAGGGG
AGCCAGGAACGGGACATTGCTTTGGCAAAAGCCATTGCAGCGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11253.69 Da
• Isoelectric Point: 6.2117

>AT125875 WP_031575560.1 NZ_CP040511:c174816-174499 [Acidithiobacillus ferrooxidans]
MEVQFRRYDTVNYLKTEDDIAHYLDAAMEDGDPALILAALGDVARARNLSQLARDAGMSRQGLDKALSGTGNPSLVTVLK
ITQALGLRLSFQPAHTSSHPSATGV

Nucleotide

• Length: 318 bp

>AT125875 NZ_CP040511:c174816-174499 [Acidithiobacillus ferrooxidans]
ATGGAAGTGCAATTCAGACGCTACGACACCGTGAATTACCTCAAGACTGAGGACGATATTGCGCACTATCTCGATGCTGC
GATGGAGGATGGCGACCCCGCGTTGATTCTTGCAGCGCTGGGAGACGTTGCCCGTGCCCGCAACCTCAGCCAGCTCGCCC
GCGACGCAGGCATGAGCCGCCAAGGATTGGACAAGGCCCTTTCCGGAACAGGGAACCCAAGCCTGGTAACGGTGCTGAAA
ATAACCCAGGCACTCGGCCTGCGGCTTTCCTTCCAACCAGCACACACAAGCAGTCACCCATCGGCTACAGGGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2W1KCH5