Detailed information of TA system TA125804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 585952..586584
• Replicon: chromosome
• Accession: NZ_CP040441
• Organism: Alkalihalobacillus halodurans isolate LB-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9KFF7
• Locus tag: FED51_RS02915
• Protein ID: WP_010896700.1
• Coordinates: 586234..586584 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FED51_RS02910
• Protein ID: WP_173355321.1
• Coordinates: 585952..586230 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FED51_RS02890	581847..582593	-	747	WP_053432219.1	rhomboid family intramembrane serine protease	-
FED51_RS02895	582815..583174	+	360	WP_010896696.1	holo-ACP synthase	-
FED51_RS02900	583361..584365	+	1005	WP_053432220.1	outer membrane lipoprotein carrier protein LolA	-
FED51_RS02905	584592..585758	+	1167	WP_053432221.1	alanine racemase	-
FED51_RS02910	585952..586230	+	279	WP_173355321.1	antitoxin	Antitoxin
FED51_RS02915	586234..586584	+	351	WP_010896700.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FED51_RS02920	586765..587580	+	816	WP_053432222.1	RsbT co-antagonist protein RsbRA	-
FED51_RS02925	587595..587951	+	357	WP_053432223.1	STAS domain-containing protein	-
FED51_RS02930	587956..588375	+	420	WP_053432224.1	anti-sigma regulatory factor	-
FED51_RS02935	588390..589403	+	1014	WP_010896704.1	PP2C family protein-serine/threonine phosphatase	-
FED51_RS02940	589475..589807	+	333	WP_053432225.1	STAS domain-containing protein	-
FED51_RS02945	589804..590292	+	489	WP_053432226.1	anti-sigma B factor RsbW	-
FED51_RS02950	590252..591037	+	786	WP_138090271.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12904.97 Da
• Isoelectric Point: 6.4662

>T125804 WP_010896700.1 NZ_CP040441:586234-586584 [Alkalihalobacillus halodurans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMSKVNDALLISLGLIDF

Nucleotide

• Length: 351 bp

>T125804 NZ_CP040441:586234-586584 [Alkalihalobacillus halodurans]
TTGATTGTAAAGCGAGGCGACGTGTACTTTGCCGACTTGTCTCCTGTCGTTGGTTCTGAACAAGGCGGAGTTCGGCCGGT
TCTCGTCATACAAAATGATATTGGAAATCGGTTCAGTCCCACTGTCATTGTGGCTGCAATCACCGCACAAATCCAAAAGG
CGAAGTTGCCGACTCATGTAGAAATTAATGCGAAGCGCTATGGTTTCGATCGCGATTCCGTTATTTTGCTTGAGCAAATT
CGAACAATTGATAAACAGAGGCTGACGGACAAGATTACCCATCTTGACGATGATATGATGTCGAAGGTAAATGATGCCCT
GCTTATTAGTTTAGGTCTAATCGACTTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10676.23 Da
• Isoelectric Point: 8.5491

>AT125804 WP_173355321.1 NZ_CP040441:585952-586230 [Alkalihalobacillus halodurans]
VSESSTKRIVVNLPQHLLNEVDGVIKQEKVNRSEFFSQATKMYLRERKKRQIREKMQQGYLEMAKINLNIASEAFLAEEE
AEHTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT125804 NZ_CP040441:585952-586230 [Alkalihalobacillus halodurans]
GTGTCAGAATCAAGCACAAAACGAATTGTCGTGAATTTACCACAACATTTGCTAAATGAAGTGGACGGCGTTATTAAACA
AGAGAAGGTTAATCGGAGTGAGTTTTTCTCTCAAGCCACAAAAATGTATTTACGTGAACGAAAAAAACGTCAAATTCGTG
AAAAAATGCAGCAAGGCTACTTAGAGATGGCGAAAATTAATCTTAATATTGCGTCTGAAGCTTTTTTGGCTGAGGAGGAA
GCTGAGCACACCCTAGATCGCTTAGTAAGTGGGGTGTAG

Structures

Toxin

• AlphaFold DB: Q9KFF7