Detailed information of TA system TA125687

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4795337..4796171
• Replicon: chromosome
• Accession: NZ_CP040390
• Organism: Escherichia coli strain A1_136

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: FE198_RS23890
• Protein ID: WP_000854820.1
• Coordinates: 4795794..4796171 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: FE198_RS23885
• Protein ID: WP_001285610.1
• Coordinates: 4795337..4795705 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE198_RS23850	4791058..4791738	+	681	WP_021515201.1	WYL domain-containing protein	-
FE198_RS23855	4791889..4792566	+	678	WP_060579144.1	hypothetical protein	-
FE198_RS23860	4792572..4792805	+	234	WP_000883181.1	DUF905 family protein	-
FE198_RS23865	4792895..4793713	+	819	WP_001175155.1	DUF945 domain-containing protein	-
FE198_RS23870	4793979..4794458	+	480	WP_000706978.1	antirestriction protein	-
FE198_RS23875	4794473..4794949	+	477	WP_001186193.1	RadC family protein	-
FE198_RS23880	4795036..4795257	+	222	WP_000692347.1	DUF987 domain-containing protein	-
FE198_RS23885	4795337..4795705	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FE198_RS23890	4795794..4796171	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FE198_RS23895	4796168..4796365	+	198	Protein_4584	hypothetical protein	-
FE198_RS23900	4796393..4796590	+	198	WP_085949158.1	DUF957 domain-containing protein	-
FE198_RS23905	4796675..4797235	+	561	Protein_4586	DUF4942 domain-containing protein	-
FE198_RS23910	4798070..4799608	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4774753..4807421	32668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T125687 WP_000854820.1 NZ_CP040390:4795794-4796171 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T125687 NZ_CP040390:4795794-4796171 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT125687 WP_001285610.1 NZ_CP040390:4795337-4795705 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT125687 NZ_CP040390:4795337-4795705 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6