Detailed information of TA system TA125414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2904332..2904803
• Replicon: chromosome
• Accession: NZ_CP040311
• Organism: Escherichia coli strain F3398

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FEL04_RS14030
• Protein ID: WP_001303511.1
• Coordinates: 2904525..2904803 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FEL04_RS14025
• Protein ID: WP_001302048.1
• Coordinates: 2904332..2904523 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL04_RS13985	2899539..2900123	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FEL04_RS13990	2900180..2900575	-	396	WP_001118159.1	DUF977 family protein	-
FEL04_RS13995	2900591..2901360	-	770	Protein_2720	DUF1627 domain-containing protein	-
FEL04_RS14000	2901386..2902126	-	741	WP_000788938.1	ATP-binding protein	-
FEL04_RS14005	2902133..2903095	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
FEL04_RS14010	2903118..2903543	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FEL04_RS14015	2903540..2903842	-	303	WP_000172738.1	transcriptional regulator	-
FEL04_RS14020	2903940..2904311	+	372	WP_001169686.1	hypothetical protein	-
FEL04_RS14025	2904332..2904523	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FEL04_RS14030	2904525..2904803	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEL04_RS14035	2905155..2905454	+	300	WP_001240334.1	hypothetical protein	-
FEL04_RS14040	2905526..2905744	+	219	WP_001171930.1	hypothetical protein	-
FEL04_RS14050	2906313..2906501	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FEL04_RS14055	2906498..2906689	+	192	WP_001090200.1	DUF1482 family protein	-
FEL04_RS14060	2906782..2909253	+	2472	WP_000048458.1	exonuclease	-
FEL04_RS14065	2909326..2909577	+	252	WP_000005552.1	excisionase family protein	-
FEL04_RS14070	2909612..2909796	+	185	Protein_2734	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2814930..2909767	94837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T125414 WP_001303511.1 NZ_CP040311:2904525-2904803 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T125414 NZ_CP040311:2904525-2904803 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT125414 WP_001302048.1 NZ_CP040311:2904332-2904523 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT125414 NZ_CP040311:2904332-2904523 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0