Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4555648..4556302 Replicon chromosome
Accession NZ_CP040307
Organism Escherichia coli strain F1 E4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FEL01_RS22600 Protein ID WP_000244781.1
Coordinates 4555648..4556055 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FEL01_RS22605 Protein ID WP_000354046.1
Coordinates 4556036..4556302 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEL01_RS22580 4551605..4553338 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
FEL01_RS22585 4553344..4554054 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FEL01_RS22590 4554079..4554975 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FEL01_RS22595 4555087..4555608 + 522 WP_001055874.1 flavodoxin FldB -
FEL01_RS22600 4555648..4556055 - 408 WP_000244781.1 protein YgfX Toxin
FEL01_RS22605 4556036..4556302 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FEL01_RS22610 4556545..4557525 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
FEL01_RS22615 4557602..4558261 - 660 WP_000250274.1 hemolysin III family protein -
FEL01_RS22620 4558425..4558736 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FEL01_RS22625 4558781..4560214 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T125339 WP_000244781.1 NZ_CP040307:c4556055-4555648 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T125339 NZ_CP040307:c4556055-4555648 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT125339 WP_000354046.1 NZ_CP040307:c4556302-4556036 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT125339 NZ_CP040307:c4556302-4556036 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References