Detailed information of TA system TA125306

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3265383..3266178
• Replicon: chromosome
• Accession: NZ_CP040305
• Organism: Escherichia coli strain HB6

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: FEL06_RS16335
• Protein ID: WP_000854914.1
• Coordinates: 3265383..3265757 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FEL06_RS16340
• Protein ID: WP_001416641.1
• Coordinates: 3265804..3266178 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEL06_RS16295	3260387..3260878	-	492	WP_001301587.1	DUF1097 domain-containing protein	-
FEL06_RS16300	3260980..3261534	-	555	WP_001001917.1	molecular chaperone YcdY	-
FEL06_RS16305	3261558..3262295	-	738	WP_000283664.1	zinc-binding phosphatase	-
FEL06_RS16310	3262350..3263288	-	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
FEL06_RS16320	3263759..3264601	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
FEL06_RS16325	3264686..3264883	-	198	WP_000839282.1	DUF957 domain-containing protein	-
FEL06_RS16330	3264895..3265386	-	492	WP_000976853.1	hypothetical protein	-
FEL06_RS16335	3265383..3265757	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
FEL06_RS16340	3265804..3266178	-	375	WP_001416641.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FEL06_RS16345	3266341..3266562	-	222	WP_001220314.1	DUF987 domain-containing protein	-
FEL06_RS16350	3266625..3267101	-	477	WP_001186738.1	RadC family protein	-
FEL06_RS16355	3267117..3267602	-	486	WP_000214398.1	antirestriction protein	-
FEL06_RS16360	3267693..3268511	-	819	WP_001234682.1	DUF945 domain-containing protein	-
FEL06_RS16365	3268851..3269921	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
FEL06_RS16370	3269918..3270823	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T125306 WP_000854914.1 NZ_CP040305:c3265757-3265383 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T125306 NZ_CP040305:c3265757-3265383 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13778.61 Da
• Isoelectric Point: 6.6249

>AT125306 WP_001416641.1 NZ_CP040305:c3266178-3265804 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT125306 NZ_CP040305:c3266178-3265804 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTTCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5