Detailed information of TA system TA124971

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2730828..2731199
• Replicon: chromosome
• Accession: NZ_CP040107
• Organism: Escherichia coli O157:H7 strain MB9-1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: FDZ86_RS13800
• Protein ID: WP_042853000.1
• Coordinates: 2731005..2731199 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2730828..2731006 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FDZ86_RS13770	2726580..2726750	+	171	WP_001625136.1	hypothetical protein	-
FDZ86_RS13775	2726783..2728156	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
FDZ86_RS13780	2728285..2729220	-	936	WP_001157382.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FDZ86_RS13785	2729272..2730507	-	1236	WP_000040845.1	site-specific integrase	-
FDZ86_RS13790	2730509..2730724	-	216	WP_000079604.1	excisionase XisR	-
FDZ86_RS13795	2730824..2731012	-	189	WP_001356607.1	DUF1187 family protein	-
-	2730828..2731006	+	179	NuclAT_0	-	Antitoxin
-	2730828..2731006	+	179	NuclAT_0	-	Antitoxin
-	2730828..2731006	+	179	NuclAT_0	-	Antitoxin
-	2730828..2731006	+	179	NuclAT_0	-	Antitoxin
FDZ86_RS13800	2731005..2731199	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FDZ86_RS13805	2731256..2732065	-	810	WP_000166315.1	recombination protein RecT	-
FDZ86_RS13810	2732058..2734727	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
FDZ86_RS13815	2734808..2734984	-	177	WP_000245534.1	hypothetical protein	-
FDZ86_RS13820	2734978..2735199	-	222	WP_000560226.1	killing protein KilR	-
FDZ86_RS13825	2735246..2736094	-	849	WP_000887681.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2729272..2772055	42783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T124971 WP_042853000.1 NZ_CP040107:c2731199-2731005 [Escherichia coli O157:H7]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T124971 NZ_CP040107:c2731199-2731005 [Escherichia coli O157:H7]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT124971 NZ_CP040107:2730828-2731006 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin