Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3485382..3486052 Replicon chromosome
Accession NZ_CP039850
Organism Mycobacterium tuberculosis variant bovis strain Danish 1331

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FCU26_RS17135 Protein ID WP_003899954.1
Coordinates 3485382..3485726 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FCU26_RS17140 Protein ID WP_003899955.1
Coordinates 3485723..3486052 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCU26_RS17100 3480455..3481315 + 861 WP_003416628.1 alpha/beta hydrolase -
FCU26_RS17105 3481290..3481805 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FCU26_RS17115 3482045..3482338 + 294 WP_003416635.1 hypothetical protein -
FCU26_RS17120 3482626..3483915 + 1290 WP_003416640.1 ATP-binding protein -
FCU26_RS17125 3484262..3484696 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FCU26_RS17130 3484699..3485151 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FCU26_RS17135 3485382..3485726 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FCU26_RS17140 3485723..3486052 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FCU26_RS17145 3486590..3486937 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FCU26_RS17150 3486934..3487554 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FCU26_RS17155 3487714..3488979 - 1266 WP_003909837.1 hypothetical protein -
FCU26_RS17160 3489147..3489356 + 210 WP_003416778.1 hypothetical protein -
FCU26_RS17170 3489603..3490637 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T124433 WP_003899954.1 NZ_CP039850:3485382-3485726 [Mycobacterium tuberculosis variant bovis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T124433 NZ_CP039850:3485382-3485726 [Mycobacterium tuberculosis variant bovis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT124433 WP_003899955.1 NZ_CP039850:3485723-3486052 [Mycobacterium tuberculosis variant bovis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT124433 NZ_CP039850:3485723-3486052 [Mycobacterium tuberculosis variant bovis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References