Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3284859..3285654 Replicon chromosome
Accession NZ_CP039837
Organism Escherichia coli O157:H7 strain USDA5905

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag E3175_RS16490 Protein ID WP_000854914.1
Coordinates 3284859..3285233 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7UP42
Locus tag E3175_RS16495 Protein ID WP_001280955.1
Coordinates 3285280..3285654 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3175_RS16450 3279863..3280354 - 492 WP_001301587.1 DUF1097 domain-containing protein -
E3175_RS16455 3280456..3281010 - 555 WP_001001917.1 molecular chaperone YcdY -
E3175_RS16460 3281034..3281771 - 738 WP_000283664.1 zinc-binding phosphatase -
E3175_RS16465 3281826..3282764 - 939 WP_000351299.1 glyoxylate/hydroxypyruvate reductase GhrA -
E3175_RS16475 3283235..3284077 - 843 WP_001280481.1 DUF4942 domain-containing protein -
E3175_RS16480 3284162..3284359 - 198 WP_000839282.1 DUF957 domain-containing protein -
E3175_RS16485 3284371..3284862 - 492 WP_000976857.1 hypothetical protein -
E3175_RS16490 3284859..3285233 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
E3175_RS16495 3285280..3285654 - 375 WP_001280955.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E3175_RS16500 3285817..3286038 - 222 WP_001220314.1 DUF987 domain-containing protein -
E3175_RS16505 3286101..3286577 - 477 WP_001186738.1 RadC family protein -
E3175_RS16510 3286593..3287078 - 486 WP_000214398.1 antirestriction protein -
E3175_RS16515 3287169..3287987 - 819 WP_001234685.1 DUF945 domain-containing protein -
E3175_RS16520 3288327..3289397 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
E3175_RS16525 3289394..3290299 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3276245..3287078 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T124354 WP_000854914.1 NZ_CP039837:c3285233-3284859 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T124354 NZ_CP039837:c3285233-3284859 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13767.59 Da        Isoelectric Point: 6.6248

>AT124354 WP_001280955.1 NZ_CP039837:c3285654-3285280 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT124354 NZ_CP039837:c3285654-3285280 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9P0D0

References