Detailed information of TA system TA124296

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37143..37413
• Replicon: plasmid pC2414-2-KPC
• Accession: NZ_CP039820
• Organism: Klebsiella pneumoniae strain C2414

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FA958_RS28405
• Protein ID: WP_001312861.1
• Coordinates: 37255..37413 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37143..37206 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA958_RS28380	32854..33381	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
FA958_RS28385	33439..33672	+	234	WP_000006003.1	DUF905 family protein	-
FA958_RS28390	33733..35756	+	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
FA958_RS28395	35825..36259	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FA958_RS28400	36256..36975	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	36987..37211	+	225	NuclAT_0	-	-
-	37143..37206	-	64	-	-	Antitoxin
FA958_RS28405	37255..37413	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FA958_RS30050	37651..38028	-	378	Protein_49	hypothetical protein	-
FA958_RS28415	38328..38624	+	297	WP_001272251.1	hypothetical protein	-
FA958_RS28420	38805..39785	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
FA958_RS28425	39935..40756	+	822	WP_001234445.1	DUF945 domain-containing protein	-
FA958_RS28430	41053..41700	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
FA958_RS28435	41977..42360	+	384	WP_000124981.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..172001	172001
	-	flank	IS/Tn	-	-	38805..39785	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T124296 WP_001312861.1 NZ_CP039820:37255-37413 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T124296 NZ_CP039820:37255-37413 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT124296 NZ_CP039820:c37206-37143 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin