Detailed information of TA system TA124165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 50162..50687
• Replicon: plasmid PNCS014858 strain 14[5]12:i:-
• Accession: NZ_CP039583
• Organism: Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain PNCS014858

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: E5O01_RS24755
• Protein ID: WP_001159863.1
• Coordinates: 50382..50687 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: E5O01_RS24750
• Protein ID: WP_000813641.1
• Coordinates: 50162..50380 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5O01_RS24720	45449..45830	+	382	Protein_49	NirD/YgiW/YdeI family stress tolerance protein	-
E5O01_RS24725	46107..46443	+	337	Protein_50	site-specific integrase	-
E5O01_RS24730	46702..47691	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
E5O01_RS24735	48185..48481	-	297	WP_001687482.1	hypothetical protein	-
E5O01_RS24745	48965..49486	-	522	WP_010999942.1	hypothetical protein	-
E5O01_RS24750	50162..50380	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E5O01_RS24755	50382..50687	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
E5O01_RS24760	50689..50979	+	291	WP_001266176.1	hypothetical protein	-
E5O01_RS24765	50976..51497	+	522	WP_000198608.1	hypothetical protein	-
E5O01_RS24770	51532..52314	+	783	WP_000082169.1	site-specific integrase	-
E5O01_RS24775	52323..53036	+	714	Protein_59	EAL domain-containing protein	-
E5O01_RS24785	53040..53549	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
E5O01_RS24790	53543..54028	+	486	WP_000905606.1	membrane protein	-
E5O01_RS24795	54305..54592	-	288	WP_071530243.1	hypothetical protein	-
E5O01_RS24800	54748..55308	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94000	94000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T124165 WP_001159863.1 NZ_CP039583:50382-50687 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T124165 NZ_CP039583:50382-50687 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT124165 WP_000813641.1 NZ_CP039583:50162-50380 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT124165 NZ_CP039583:50162-50380 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6