Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16657..17182 Replicon plasmid pHB25-1-88K
Accession NZ_CP039525
Organism Klebsiella pneumoniae subsp. pneumoniae strain HB25-1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag E8Q43_RS27405 Protein ID WP_025714517.1
Coordinates 16877..17182 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag E8Q43_RS27400 Protein ID WP_001568025.1
Coordinates 16657..16875 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E8Q43_RS27390 14339..15367 - 1029 WP_001568023.1 Abi family protein -
E8Q43_RS27395 15508..16077 - 570 WP_016162066.1 hypothetical protein -
E8Q43_RS27400 16657..16875 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E8Q43_RS27405 16877..17182 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
E8Q43_RS27410 17351..17746 + 396 WP_017899885.1 hypothetical protein -
E8Q43_RS27415 17773..18087 + 315 WP_053389906.1 hypothetical protein -
E8Q43_RS27420 18098..19114 + 1017 WP_017899884.1 hypothetical protein -
E8Q43_RS27430 19312..20106 + 795 WP_004197635.1 site-specific integrase -
E8Q43_RS30195 20594..20896 - 303 WP_071571079.1 hypothetical protein -
E8Q43_RS27440 20893..21519 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88581 88581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T124089 WP_025714517.1 NZ_CP039525:16877-17182 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T124089 NZ_CP039525:16877-17182 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT124089 WP_001568025.1 NZ_CP039525:16657-16875 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT124089 NZ_CP039525:16657-16875 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References