Detailed information of TA system TA123896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3593007..3593734
• Replicon: chromosome
• Accession: NZ_CP039403
• Organism: Escherichia coli strain 382634_2f

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: E4311_RS17795
• Protein ID: WP_000547563.1
• Coordinates: 3593423..3593734 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E4311_RS17790
• Protein ID: WP_000126304.1
• Coordinates: 3593007..3593426 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4311_RS17770	3589170..3591422	-	2253	WP_001592558.1	carbamoyltransferase HypF	-
E4311_RS17775	3591575..3592102	-	528	WP_001078777.1	electron transport protein HydN	-
E4311_RS17785	3592487..3592915	+	429	WP_000536064.1	hypothetical protein	-
E4311_RS17790	3593007..3593426	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
E4311_RS17795	3593423..3593734	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
E4311_RS17800	3593897..3594652	-	756	WP_001374644.1	hypothetical protein	-
E4311_RS17805	3594695..3595162	-	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
E4311_RS17810	3595155..3595565	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
E4311_RS17815	3595562..3596329	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
E4311_RS17820	3596329..3596871	-	543	WP_000493787.1	formate hydrogenlyase subunit HycF	-
E4311_RS17825	3596881..3598590	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T123896 WP_000547563.1 NZ_CP039403:c3593734-3593423 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T123896 NZ_CP039403:c3593734-3593423 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT123896 WP_000126304.1 NZ_CP039403:c3593426-3593007 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT123896 NZ_CP039403:c3593426-3593007 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22