Detailed information of TA system TA123671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 17746..18389
• Replicon: plasmid unnamed2
• Accession: NZ_CP039206
• Organism: Piscirickettsia salmonis strain Psal-182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal182_RS17265
• Protein ID: WP_016210974.1
• Coordinates: 17982..18389 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal182_RS17260
• Protein ID: WP_016210972.1
• Coordinates: 17746..17973 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal182_RS17210	13141..13443	+	303	WP_036780299.1	hypothetical protein	-
Psal182_RS17215	13482..14468	+	987	WP_016211080.1	helix-turn-helix domain-containing protein	-
Psal182_RS17220	14509..15045	+	537	WP_155085020.1	hypothetical protein	-
Psal182_RS17225	15014..15376	+	363	WP_016210959.1	hypothetical protein	-
Psal182_RS17230	15369..15716	+	348	WP_016210966.1	hypothetical protein	-
Psal182_RS17235	15713..15952	+	240	WP_016210963.1	hypothetical protein	-
Psal182_RS17240	16048..16335	+	288	WP_155085013.1	hypothetical protein	-
Psal182_RS17245	16356..16616	+	261	WP_016210965.1	hypothetical protein	-
Psal182_RS17250	16618..17091	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal182_RS17255	17206..17598	+	393	WP_129556726.1	hypothetical protein	-
Psal182_RS17260	17746..17973	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal182_RS17265	17982..18389	+	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal182_RS17270	18506..19186	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal182_RS17275	19365..19658	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal182_RS17280	19876..20058	-	183	WP_155047026.1	hypothetical protein	-
Psal182_RS17285	20203..20382	-	180	WP_155046773.1	phosphatase	-
Psal182_RS17290	20477..20794	+	318	WP_129556723.1	hypothetical protein	-
Psal182_RS17295	21095..21478	+	384	WP_032126134.1	HNH endonuclease	-
Psal182_RS17300	21566..22018	+	453	WP_155085014.1	P27 family phage terminase small subunit	-
Psal182_RS17305	22055..22783	+	729	WP_054300202.1	IS6 family transposase	-
Psal182_RS17310	22928..23206	-	279	WP_129556745.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53285	53285
	-	flank	IS/Tn	-	-	22055..22783	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123671 WP_016210974.1 NZ_CP039206:17982-18389 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123671 NZ_CP039206:17982-18389 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123671 WP_016210972.1 NZ_CP039206:17746-17973 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123671 NZ_CP039206:17746-17973 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5