Detailed information of TA system TA123669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 102872..103408
• Replicon: plasmid unnamed1
• Accession: NZ_CP039205
• Organism: Piscirickettsia salmonis strain Psal-182

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6I5YBD1
• Locus tag: Psal182_RS16815
• Protein ID: WP_016211910.1
• Coordinates: 103136..103408 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: Psal182_RS16810
• Protein ID: WP_032126795.1
• Coordinates: 102872..103132 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal182_RS16775	98610..98936	-	327	WP_016212298.1	hypothetical protein	-
Psal182_RS16780	99177..99653	+	477	WP_051307374.1	Clp protease ClpP	-
Psal182_RS16785	99768..100061	+	294	WP_032126637.1	transposase	-
Psal182_RS16790	100022..100702	+	681	Protein_131	IS3 family transposase	-
Psal182_RS16795	100907..101209	+	303	WP_098082791.1	IS3 family transposase	-
Psal182_RS16800	101218..101919	-	702	WP_155085003.1	IS6 family transposase	-
Psal182_RS16805	102009..102599	-	591	WP_016211912.1	site-specific integrase	-
Psal182_RS16810	102872..103132	+	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Psal182_RS16815	103136..103408	+	273	WP_016211910.1	Txe/YoeB family addiction module toxin	Toxin
Psal182_RS16820	103734..104855	-	1122	WP_016211913.1	hypothetical protein	-
Psal182_RS16825	105288..105548	+	261	WP_155085004.1	helix-turn-helix domain-containing protein	-
Psal182_RS16830	105569..105862	+	294	WP_032126637.1	transposase	-
Psal182_RS16835	105945..106502	-	558	WP_081377915.1	IS3 family transposase	-
Psal182_RS16840	106647..106976	-	330	WP_081377914.1	IS3 family transposase	-
Psal182_RS16845	107093..107386	-	294	WP_155085005.1	transposase	-
Psal182_RS16850	107446..108048	+	603	Protein_143	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..144508	144508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10739.22 Da
• Isoelectric Point: 7.8926

>T123669 WP_016211910.1 NZ_CP039205:103136-103408 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Nucleotide

• Length: 273 bp

>T123669 NZ_CP039205:103136-103408 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
ACTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCACGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT123669 WP_032126795.1 NZ_CP039205:102872-103132 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT123669 NZ_CP039205:102872-103132 [Piscirickettsia salmonis]
ATGTTACGAGACAACATTAATGCTACGGCAGCGCGGGATAATTTTAAGAGTACCTGTGATACGGTGTGTGAAAACAATGC
CCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6I5YBD1

Antitoxin

• AlphaFold DB: A0A0B8U9U2