Detailed information of TA system TA123668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 50105..50675
• Replicon: plasmid unnamed1
• Accession: NZ_CP039205
• Organism: Piscirickettsia salmonis strain Psal-182

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal182_RS16450
• Protein ID: WP_036781349.1
• Coordinates: 50349..50675 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal182_RS16445
• Protein ID: WP_016212365.1
• Coordinates: 50105..50347 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal182_RS16425	45590..47275	-	1686	WP_075275141.1	protein kinase	-
Psal182_RS16430	47558..48286	-	729	WP_075274745.1	IS6-like element ISPsa2 family transposase	-
Psal182_RS16435	48354..48794	-	441	Protein_61	tyrosine-type recombinase/integrase	-
Psal182_RS16440	48922..49896	-	975	WP_054300271.1	IS30 family transposase	-
Psal182_RS16445	50105..50347	+	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
Psal182_RS16450	50349..50675	+	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal182_RS16455	50791..51519	+	729	WP_054300202.1	IS6 family transposase	-
Psal182_RS16460	51989..52372	+	384	WP_016212152.1	hypothetical protein	-
Psal182_RS16465	52679..53056	-	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
Psal182_RS16470	53221..53553	-	333	WP_032126739.1	transposase	-
Psal182_RS16480	53742..54521	-	780	WP_081377351.1	transposase	-
Psal182_RS16485	54622..54999	-	378	Protein_70	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..144508	144508
	-	inside	IScluster/Tn	-	-	43850..71603	27753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T123668 WP_036781349.1 NZ_CP039205:50349-50675 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123668 NZ_CP039205:50349-50675 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTGACTTCCAAGCCCTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGAGCAGTGTTGGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGGCTGGAATCATGCTGATAAGCTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGCTAATTGATGTGATGTTGAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT123668 WP_016212365.1 NZ_CP039205:50105-50347 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123668 NZ_CP039205:50105-50347 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGGAATAGCTTGGGGTTGTTGCTACCCTTGAGCATCGCACGTGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACTCATACGCAGACCCTTGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA