Detailed information of TA system TA123485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 15226..15869
• Replicon: plasmid unnamed3
• Accession: NZ_CP039068
• Organism: Piscirickettsia salmonis strain Psal-107

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal107_RS17140
• Protein ID: WP_016210974.1
• Coordinates: 15226..15633 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal107_RS17145
• Protein ID: WP_016210972.1
• Coordinates: 15642..15869 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal107_RS17105	11561..12040	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal107_RS17110	12128..12511	-	384	WP_032126134.1	HNH endonuclease	-
Psal107_RS17115	12821..13138	-	318	WP_129556723.1	hypothetical protein	-
Psal107_RS17120	13233..13412	+	180	WP_155046773.1	phosphatase	-
Psal107_RS17125	13557..13739	+	183	WP_155047026.1	hypothetical protein	-
Psal107_RS17130	13957..14250	-	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal107_RS17135	14429..15109	-	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal107_RS17140	15226..15633	-	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal107_RS17145	15642..15869	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal107_RS17150	16006..16980	-	975	WP_054300271.1	IS30 family transposase	-
Psal107_RS17155	17206..18297	-	1092	WP_054300578.1	hypothetical protein	-
Psal107_RS17160	18828..19292	-	465	WP_016212274.1	type II toxin-antitoxin system HicB family antitoxin	-
Psal107_RS17165	19303..19497	-	195	WP_032126154.1	type II toxin-antitoxin system HicA family toxin	-
Psal107_RS17170	19713..20303	+	591	WP_032126152.1	site-specific integrase	-
Psal107_RS17175	20367..20708	+	342	WP_081007075.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39335	39335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123485 WP_016210974.1 NZ_CP039068:c15633-15226 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123485 NZ_CP039068:c15633-15226 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123485 WP_016210972.1 NZ_CP039068:c15869-15642 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123485 NZ_CP039068:c15869-15642 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5