Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2063012..2063514 Replicon chromosome
Accession NZ_CP038791
Organism Escherichia coli strain PF9285

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E5M00_RS10130 Protein ID WP_000767829.1
Coordinates 2063260..2063514 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E5M00_RS10125 Protein ID WP_001259255.1
Coordinates 2063012..2063263 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5M00_RS10100 2058190..2059257 - 1068 WP_000080142.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
E5M00_RS10105 2059257..2060327 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
E5M00_RS10110 2060324..2061628 - 1305 WP_001355813.1 histidinol dehydrogenase -
E5M00_RS10115 2061634..2062533 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
E5M00_RS10120 2062679..2062729 - 51 WP_001364200.1 his operon leader peptide -
E5M00_RS10125 2063012..2063263 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E5M00_RS10130 2063260..2063514 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E5M00_RS10135 2063597..2064421 + 825 WP_000754737.1 SDR family oxidoreductase -
E5M00_RS10140 2064467..2065396 + 930 WP_000803360.1 LysR family transcriptional regulator -
E5M00_RS10145 2065611..2065673 + 63 WP_010723108.1 membrane protein YoeI -
E5M00_RS10150 2065663..2067021 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E5M00_RS10155 2067200..2068258 + 1059 WP_032223502.1 transport protein YeeE -
E5M00_RS10160 2068272..2068499 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T123129 WP_000767829.1 NZ_CP038791:2063260-2063514 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T123129 NZ_CP038791:2063260-2063514 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT123129 WP_001259255.1 NZ_CP038791:2063012-2063263 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT123129 NZ_CP038791:2063012-2063263 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References