Detailed information of TA system TA123045

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3213673..3214517
• Replicon: chromosome
• Accession: NZ_CP038505
• Organism: Escherichia coli strain 28Eco12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: DC440_RS16160
• Protein ID: WP_000854686.1
• Coordinates: 3213673..3214056 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: DC440_RS16165
• Protein ID: WP_001285602.1
• Coordinates: 3214137..3214517 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DC440_RS16120	3208675..3209166	-	492	WP_032145787.1	DUF1097 domain-containing protein	-
DC440_RS16125	3209268..3209822	-	555	WP_001001909.1	molecular chaperone YcdY	-
DC440_RS16130	3209846..3210583	-	738	WP_000283664.1	zinc-binding phosphatase	-
DC440_RS16135	3210638..3211576	-	939	WP_001550942.1	glyoxylate/hydroxypyruvate reductase GhrA	-
DC440_RS16145	3212047..3212889	-	843	WP_001431817.1	DUF4942 domain-containing protein	-
DC440_RS16150	3212974..3213171	-	198	WP_000839253.1	DUF957 domain-containing protein	-
DC440_RS16155	3213188..3213676	-	489	WP_001054233.1	hypothetical protein	-
DC440_RS16160	3213673..3214056	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
DC440_RS16165	3214137..3214517	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DC440_RS16170	3214528..3215211	-	684	WP_000086768.1	hypothetical protein	-
DC440_RS16175	3215230..3215451	-	222	WP_000692298.1	DUF987 domain-containing protein	-
DC440_RS16180	3215514..3215990	-	477	WP_001186726.1	RadC family protein	-
DC440_RS16185	3216006..3216491	-	486	WP_000214307.1	antirestriction protein	-
DC440_RS16190	3216583..3217401	-	819	WP_001234732.1	DUF945 domain-containing protein	-
DC440_RS16200	3217501..3217734	-	234	WP_001119719.1	DUF905 family protein	-
DC440_RS16205	3217813..3218268	-	456	WP_001504120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T123045 WP_000854686.1 NZ_CP038505:c3214056-3213673 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T123045 NZ_CP038505:c3214056-3213673 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT123045 WP_001285602.1 NZ_CP038505:c3214517-3214137 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT123045 NZ_CP038505:c3214517-3214137 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG