Detailed information of TA system TA122668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3467485..3467710
• Replicon: chromosome
• Accession: NZ_CP038421
• Organism: Escherichia coli O157:H7 strain 7636

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3152_RS17765
• Protein ID: WP_000813263.1
• Coordinates: 3467485..3467640 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3467652..3467710 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3152_RS17730	3462939..3463652	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E3152_RS17735	3463790..3463986	-	197	Protein_3474	TrmB family transcriptional regulator	-
E3152_RS17740	3464273..3465091	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3152_RS17745	3465243..3465614	-	372	WP_000090264.1	antitermination protein	-
E3152_RS17750	3465604..3465975	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3152_RS17755	3465988..3467037	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E3152_RS17760	3467039..3467317	-	279	WP_001341388.1	hypothetical protein	-
E3152_RS17765	3467485..3467640	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3467652..3467710	+	59	-	-	Antitoxin
E3152_RS17770	3467742..3467879	+	138	WP_000955173.1	hypothetical protein	-
E3152_RS17775	3468245..3469018	+	774	WP_000160654.1	alpha/beta hydrolase	-
E3152_RS17780	3469370..3469783	-	414	WP_001151233.1	DUF977 family protein	-
E3152_RS17785	3469799..3470569	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3152_RS17790	3470591..3471337	-	747	WP_000788751.1	ATP-binding protein	-
E3152_RS17795	3471344..3472435	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122668 WP_000813263.1 NZ_CP038421:c3467640-3467485 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122668 NZ_CP038421:c3467640-3467485 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122668 NZ_CP038421:3467652-3467710 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin