Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 74767..75326 | Replicon | plasmid pBB24-1 |
| Accession | NZ_CP038404 | ||
| Organism | Escherichia coli O157:H7 strain BB24-1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E4U87_RS28415 | Protein ID | WP_000421248.1 |
| Coordinates | 75051..75326 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E4U87_RS28410 | Protein ID | WP_001178089.1 |
| Coordinates | 74767..75051 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U87_RS28370 | 69959..70519 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| E4U87_RS28375 | 70652..70864 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E4U87_RS28380 | 71109..71570 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| E4U87_RS28385 | 71616..71825 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E4U87_RS28390 | 71863..72201 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| E4U87_RS28395 | 72441..72695 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E4U87_RS28400 | 72931..73005 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E4U87_RS28405 | 72998..73855 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E4U87_RS28410 | 74767..75051 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E4U87_RS28415 | 75051..75326 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U87_RS28420 | 75421..75627 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| E4U87_RS28425 | 75727..75942 | - | 216 | Protein_82 | transposase | - |
| E4U87_RS28430 | 75986..76552 | - | 567 | Protein_83 | IS3-like element IS600 family transposase | - |
| E4U87_RS28435 | 76552..76968 | - | 417 | Protein_84 | transposase zinc-binding domain-containing protein | - |
| E4U87_RS28440 | 76968..77153 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| E4U87_RS28445 | 77330..79540 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| E4U87_RS28450 | 79584..79973 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP | 1..95604 | 95604 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T122426 WP_000421248.1 NZ_CP038404:75051-75326 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T122426 NZ_CP038404:75051-75326 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT122426 WP_001178089.1 NZ_CP038404:74767-75051 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT122426 NZ_CP038404:74767-75051 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |