Detailed information of TA system TA122366

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2538487..2538712
• Replicon: chromosome
• Accession: NZ_CP038398
• Organism: Escherichia coli O157:H7 strain DEC4E

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3154_RS12670
• Protein ID: WP_000935259.1
• Coordinates: 2538500..2538712 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2538487..2538545 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3154_RS12620	2533552..2533794	+	243	WP_000747948.1	hypothetical protein	-
E3154_RS12625	2533778..2534203	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E3154_RS12630	2534272..2535315	+	1044	WP_001262402.1	hypothetical protein	-
E3154_RS12635	2535308..2535769	+	462	WP_000139447.1	replication protein	-
E3154_RS12640	2535803..2536519	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3154_RS12645	2536552..2536833	+	282	WP_000603384.1	DNA-binding protein	-
E3154_RS12650	2536830..2537057	+	228	WP_000699809.1	hypothetical protein	-
E3154_RS12655	2537050..2537361	+	312	WP_001289673.1	hypothetical protein	-
E3154_RS12660	2537489..2537707	+	219	WP_000683609.1	DUF4014 family protein	-
E3154_RS12665	2537709..2538266	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2538487..2538545	-	59	-	-	Antitoxin
E3154_RS12670	2538500..2538712	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3154_RS12675	2538832..2539176	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3154_RS12680	2539298..2539570	+	273	WP_000191872.1	hypothetical protein	-
E3154_RS12685	2539572..2540621	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3154_RS12690	2540634..2540939	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E3154_RS12695	2541002..2541556	+	555	WP_000640035.1	DUF1133 family protein	-
E3154_RS12700	2541781..2541978	+	198	WP_000917763.1	hypothetical protein	-
E3154_RS12705	2542114..2542827	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3154_RS12720	2543278..2543709	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2485671..2575449	89778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122366 WP_000935259.1 NZ_CP038398:2538500-2538712 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122366 NZ_CP038398:2538500-2538712 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122366 NZ_CP038398:c2538545-2538487 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin