Detailed information of TA system TA122333

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2239422..2239793
• Replicon: chromosome
• Accession: NZ_CP038394
• Organism: Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A368IS51
• Locus tag: E3155_RS10920
• Protein ID: WP_071780718.1
• Coordinates: 2239599..2239793 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2239422..2239600 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3155_RS10880	2234864..2235520	-	657	WP_000011661.1	carbon-nitrogen hydrolase family protein	-
E3155_RS10885	2235622..2235852	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
E3155_RS10890	2235991..2236365	+	375	WP_000168751.1	CopC domain-containing protein YobA	-
E3155_RS10895	2236369..2237241	+	873	WP_000879314.1	copper homeostasis membrane protein CopD	-
E3155_RS10900	2237254..2237595	+	342	WP_000976483.1	YebY family protein	-
E3155_RS10905	2237988..2239064	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
E3155_RS10910	2239030..2239311	-	282	WP_001311878.1	excisionase	-
E3155_RS10915	2239418..2239606	-	189	WP_001417978.1	DUF1187 family protein	-
-	2239422..2239600	+	179	NuclAT_1	-	Antitoxin
-	2239422..2239600	+	179	NuclAT_1	-	Antitoxin
-	2239422..2239600	+	179	NuclAT_1	-	Antitoxin
-	2239422..2239600	+	179	NuclAT_1	-	Antitoxin
E3155_RS10920	2239599..2239793	-	195	WP_071780718.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E3155_RS10925	2239857..2240909	-	1053	WP_001004419.1	recombinase RecT	-
E3155_RS10930	2240921..2244067	-	3147	WP_000102214.1	exodeoxyribonuclease VIII	-
E3155_RS10935	2244167..2244442	-	276	WP_001417976.1	hypothetical protein	-
E3155_RS10940	2244517..2244693	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2232121..2296436	64315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.97 Da
• Isoelectric Point: 9.6161

>T122333 WP_071780718.1 NZ_CP038394:c2239793-2239599 [Escherichia coli O157:H7]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALARWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T122333 NZ_CP038394:c2239793-2239599 [Escherichia coli O157:H7]
ATGCGGTATGAAAAAGTCAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGGTGCGAATCCCGAACCGGCTATAGTGGAAGTGAAAAAGAAGCACTTGCAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT122333 NZ_CP038394:2239422-2239600 [Escherichia coli O157:H7]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGTGTGCCACCTTCCATTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A368IS51

Antitoxin