Detailed information of TA system TA122165

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2594417..2594642
• Replicon: chromosome
• Accession: NZ_CP038376
• Organism: Escherichia coli O157:H7 strain F1273

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3159_RS12840
• Protein ID: WP_000935259.1
• Coordinates: 2594430..2594642 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2594417..2594475 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3159_RS12790	2589482..2589724	+	243	WP_000747948.1	hypothetical protein	-
E3159_RS12795	2589708..2590133	+	426	WP_000693878.1	Rha family transcriptional regulator	-
E3159_RS12800	2590202..2591245	+	1044	WP_128484576.1	phage replisome organizer	-
E3159_RS12805	2591238..2591699	+	462	WP_000139447.1	replication protein	-
E3159_RS12810	2591733..2592449	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
E3159_RS12815	2592482..2592763	+	282	WP_000603384.1	DNA-binding protein	-
E3159_RS12820	2592760..2592987	+	228	WP_000699809.1	hypothetical protein	-
E3159_RS12825	2592980..2593291	+	312	WP_001289673.1	hypothetical protein	-
E3159_RS12830	2593419..2593637	+	219	WP_000683609.1	DUF4014 family protein	-
E3159_RS12835	2593639..2594196	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2594417..2594475	-	59	-	-	Antitoxin
E3159_RS12840	2594430..2594642	+	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3159_RS12845	2594762..2595106	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
E3159_RS12850	2595228..2595500	+	273	WP_000191872.1	hypothetical protein	-
E3159_RS12855	2595502..2596551	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
E3159_RS12860	2596564..2596869	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
E3159_RS12865	2596932..2597486	+	555	WP_000640035.1	DUF1133 family protein	-
E3159_RS12870	2597711..2597908	+	198	WP_000917763.1	hypothetical protein	-
E3159_RS12875	2598044..2598757	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
E3159_RS12890	2599208..2599639	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1 / nleG7'	2473760..2640105	166345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T122165 WP_000935259.1 NZ_CP038376:2594430-2594642 [Escherichia coli O157:H7]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T122165 NZ_CP038376:2594430-2594642 [Escherichia coli O157:H7]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122165 NZ_CP038376:c2594475-2594417 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin