Detailed information of TA system TA122163

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2546435..2546660
• Replicon: chromosome
• Accession: NZ_CP038376
• Organism: Escherichia coli O157:H7 strain F1273

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3159_RS12530
• Protein ID: WP_000967408.1
• Coordinates: 2546448..2546660 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2546435..2546493 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3159_RS12485	2541688..2542641	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E3159_RS12490	2542648..2543388	+	741	WP_000790456.1	ATP-binding protein	-
E3159_RS12495	2543418..2544188	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E3159_RS12500	2544204..2544599	+	396	WP_001118161.1	DUF977 family protein	-
E3159_RS12505	2544656..2545012	+	357	WP_001302146.1	hypothetical protein	-
E3159_RS12510	2545061..2545273	+	213	WP_000063625.1	hypothetical protein	-
E3159_RS12515	2545309..2545680	+	372	WP_000137941.1	hypothetical protein	-
E3159_RS12520	2545677..2546039	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E3159_RS12525	2546155..2546259	+	105	WP_001278450.1	hypothetical protein	-
-	2546435..2546493	-	59	-	-	Antitoxin
E3159_RS12530	2546448..2546660	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E3159_RS12535	2546957..2547124	+	168	WP_000998188.1	hypothetical protein	-
E3159_RS12540	2547190..2547468	+	279	WP_001304183.1	hypothetical protein	-
E3159_RS12545	2547470..2548519	+	1050	Protein_2436	DUF968 domain-containing protein	-
E3159_RS12550	2548532..2548906	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E3159_RS12555	2548903..2549724	+	822	WP_000762904.1	antitermination protein	-
E3159_RS12560	2549951..2550148	+	198	WP_000917735.1	hypothetical protein	-
E3159_RS12565	2550299..2551357	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1 / nleG7'	2473760..2640105	166345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T122163 WP_000967408.1 NZ_CP038376:2546448-2546660 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T122163 NZ_CP038376:2546448-2546660 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122163 NZ_CP038376:c2546493-2546435 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin