Detailed information of TA system TA122158

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1979527..1979752
• Replicon: chromosome
• Accession: NZ_CP038376
• Organism: Escherichia coli O157:H7 strain F1273

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E3159_RS09295
• Protein ID: WP_000813263.1
• Coordinates: 1979597..1979752 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1979527..1979585 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3159_RS09265	1974802..1975893	+	1092	WP_128484534.1	DNA-binding protein	-
E3159_RS09270	1975900..1976646	+	747	WP_000788751.1	ATP-binding protein	-
E3159_RS09275	1976668..1977438	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3159_RS09280	1977454..1977867	+	414	WP_001151233.1	DUF977 family protein	-
E3159_RS09285	1978219..1978992	-	774	WP_000160654.1	alpha/beta hydrolase	-
E3159_RS09290	1979358..1979495	-	138	WP_000955173.1	hypothetical protein	-
-	1979527..1979585	-	59	-	-	Antitoxin
E3159_RS09295	1979597..1979752	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
E3159_RS09300	1979920..1980198	+	279	WP_001341388.1	hypothetical protein	-
E3159_RS09305	1980200..1981249	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
E3159_RS09310	1981262..1981633	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E3159_RS09315	1981623..1981994	+	372	WP_000090264.1	antitermination protein	-
E3159_RS09320	1982146..1982964	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E3159_RS09325	1983251..1983447	+	197	Protein_1806	TrmB family transcriptional regulator	-
E3159_RS09330	1983585..1984298	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T122158 WP_000813263.1 NZ_CP038376:1979597-1979752 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T122158 NZ_CP038376:1979597-1979752 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT122158 NZ_CP038376:c1979585-1979527 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin