Detailed information of TA system TA121960

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3473743..3473968
• Replicon: chromosome
• Accession: NZ_CP038360
• Organism: Escherichia coli O157:H7 strain F7386

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U67_RS17775
• Protein ID: WP_000813263.1
• Coordinates: 3473743..3473898 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3473910..3473968 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U67_RS17740	3469197..3469910	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
E4U67_RS17745	3470048..3470244	-	197	Protein_3473	TrmB family transcriptional regulator	-
E4U67_RS17750	3470531..3471349	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
E4U67_RS17755	3471501..3471872	-	372	WP_000090264.1	antitermination protein	-
E4U67_RS17760	3471862..3472233	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
E4U67_RS17765	3472246..3473295	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
E4U67_RS17770	3473297..3473575	-	279	WP_001341388.1	hypothetical protein	-
E4U67_RS17775	3473743..3473898	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3473910..3473968	+	59	-	-	Antitoxin
E4U67_RS17780	3474000..3474137	+	138	WP_000955173.1	hypothetical protein	-
E4U67_RS17785	3474503..3475276	+	774	WP_000160654.1	alpha/beta hydrolase	-
E4U67_RS17790	3475628..3476041	-	414	WP_001151233.1	DUF977 family protein	-
E4U67_RS17795	3476057..3476827	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U67_RS17800	3476849..3477595	-	747	WP_000788745.1	ATP-binding protein	-
E4U67_RS17805	3477602..3478693	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T121960 WP_000813263.1 NZ_CP038360:c3473898-3473743 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T121960 NZ_CP038360:c3473898-3473743 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121960 NZ_CP038360:3473910-3473968 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin