Detailed information of TA system TA121888

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2960167..2960392
• Replicon: chromosome
• Accession: NZ_CP038355
• Organism: Escherichia coli O157:H7 str. F8092B

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U83_RS14860
• Protein ID: WP_000967408.1
• Coordinates: 2960167..2960379 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2960334..2960392 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U83_RS14825	2955470..2956528	-	1059	WP_000483497.1	site-specific DNA-methyltransferase	-
E4U83_RS14830	2956679..2956876	-	198	WP_000917735.1	hypothetical protein	-
E4U83_RS14835	2957103..2957924	-	822	WP_000762904.1	antitermination protein	-
E4U83_RS14840	2957921..2958295	-	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U83_RS14845	2958308..2959357	-	1050	Protein_2905	DUF968 domain-containing protein	-
E4U83_RS14850	2959359..2959637	-	279	WP_001304183.1	hypothetical protein	-
E4U83_RS14855	2959703..2959870	-	168	WP_000998188.1	hypothetical protein	-
E4U83_RS14860	2960167..2960379	-	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2960334..2960392	+	59	-	-	Antitoxin
E4U83_RS14865	2960568..2960672	-	105	WP_001278450.1	hypothetical protein	-
E4U83_RS14870	2960788..2961150	-	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U83_RS14875	2961147..2961518	-	372	WP_000137941.1	hypothetical protein	-
E4U83_RS14880	2961554..2961766	-	213	WP_000063625.1	hypothetical protein	-
E4U83_RS14885	2961815..2962171	-	357	WP_001302146.1	hypothetical protein	-
E4U83_RS14890	2962228..2962623	-	396	WP_001118161.1	DUF977 family protein	-
E4U83_RS14895	2962639..2963409	-	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U83_RS14900	2963439..2964179	-	741	WP_000790456.1	ATP-binding protein	-
E4U83_RS14905	2964186..2965139	-	954	WP_000095667.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / espM1 / nleA/espI / paa	2875121..3034105	158984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121888 WP_000967408.1 NZ_CP038355:c2960379-2960167 [Escherichia coli O157:H7 str. F8092B]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121888 NZ_CP038355:c2960379-2960167 [Escherichia coli O157:H7 str. F8092B]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121888 NZ_CP038355:2960334-2960392 [Escherichia coli O157:H7 str. F8092B]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin