Detailed information of TA system TA121549

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2598413..2598784
• Replicon: chromosome
• Accession: NZ_CP038333
• Organism: Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: E4U68_RS13300
• Protein ID: WP_021500490.1
• Coordinates: 2598590..2598784 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2598413..2598591 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U68_RS13270	2594164..2594334	+	171	WP_001625136.1	hypothetical protein	-
E4U68_RS13275	2594367..2595740	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
E4U68_RS13280	2595869..2596804	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
E4U68_RS13285	2596857..2598092	-	1236	WP_000040838.1	site-specific integrase	-
E4U68_RS13290	2598094..2598309	-	216	WP_000079604.1	excisionase XisR	-
E4U68_RS13295	2598409..2598597	-	189	WP_001298826.1	DUF1187 family protein	-
-	2598413..2598591	+	179	NuclAT_0	-	Antitoxin
-	2598413..2598591	+	179	NuclAT_0	-	Antitoxin
-	2598413..2598591	+	179	NuclAT_0	-	Antitoxin
-	2598413..2598591	+	179	NuclAT_0	-	Antitoxin
E4U68_RS13300	2598590..2598784	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
E4U68_RS13305	2598848..2599900	-	1053	WP_001004423.1	recombinase RecT	-
E4U68_RS13310	2599912..2603037	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
E4U68_RS13315	2603139..2603414	-	276	WP_171880296.1	hypothetical protein	-
E4U68_RS13320	2603489..2603665	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2582221..2644556	62335

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T121549 WP_021500490.1 NZ_CP038333:c2598784-2598590 [Escherichia coli O157:H7]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T121549 NZ_CP038333:c2598784-2598590 [Escherichia coli O157:H7]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT121549 NZ_CP038333:2598413-2598591 [Escherichia coli O157:H7]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin