Detailed information of TA system TA121129

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2596985..2597210
• Replicon: chromosome
• Accession: NZ_CP038292
• Organism: Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U65_RS13125
• Protein ID: WP_000967408.1
• Coordinates: 2596998..2597210 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2596985..2597043 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U65_RS13080	2592238..2593191	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E4U65_RS13085	2593198..2593938	+	741	WP_000790456.1	ATP-binding protein	-
E4U65_RS13090	2593968..2594738	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U65_RS13095	2594754..2595149	+	396	WP_001118161.1	DUF977 family protein	-
E4U65_RS13100	2595206..2595562	+	357	WP_001302146.1	hypothetical protein	-
E4U65_RS13105	2595611..2595823	+	213	WP_000063625.1	hypothetical protein	-
E4U65_RS13110	2595859..2596230	+	372	WP_000137941.1	hypothetical protein	-
E4U65_RS13115	2596227..2596589	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U65_RS13120	2596705..2596809	+	105	WP_001278450.1	hypothetical protein	-
-	2596985..2597043	-	59	-	-	Antitoxin
E4U65_RS13125	2596998..2597210	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U65_RS13130	2597507..2597674	+	168	WP_000998188.1	hypothetical protein	-
E4U65_RS13135	2597740..2598018	+	279	WP_001304183.1	hypothetical protein	-
E4U65_RS13140	2598020..2599069	+	1050	Protein_2566	DUF968 domain-containing protein	-
E4U65_RS13145	2599082..2599456	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U65_RS13150	2599453..2600274	+	822	WP_000762905.1	antitermination protein	-
E4U65_RS13155	2600501..2600698	+	198	WP_000917735.1	hypothetical protein	-
E4U65_RS13160	2600849..2601907	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / espX7/nleL / espN / espK	2526090..2727899	201809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121129 WP_000967408.1 NZ_CP038292:2596998-2597210 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121129 NZ_CP038292:2596998-2597210 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121129 NZ_CP038292:c2597043-2596985 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin