Detailed information of TA system TA121044

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2587220..2587445
• Replicon: chromosome
• Accession: NZ_CP038287
• Organism: Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: E4U64_RS13025
• Protein ID: WP_000967408.1
• Coordinates: 2587233..2587445 (+)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2587220..2587278 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U64_RS12980	2582473..2583426	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
E4U64_RS12985	2583433..2584173	+	741	WP_000790456.1	ATP-binding protein	-
E4U64_RS12990	2584203..2584973	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
E4U64_RS12995	2584989..2585384	+	396	WP_001118161.1	DUF977 family protein	-
E4U64_RS13000	2585441..2585797	+	357	WP_001302146.1	hypothetical protein	-
E4U64_RS13005	2585846..2586058	+	213	WP_000063625.1	hypothetical protein	-
E4U64_RS13010	2586094..2586465	+	372	WP_000137941.1	hypothetical protein	-
E4U64_RS13015	2586462..2586824	+	363	WP_000610379.1	DUF551 domain-containing protein	-
E4U64_RS13020	2586940..2587044	+	105	WP_001278450.1	hypothetical protein	-
-	2587220..2587278	-	59	-	-	Antitoxin
E4U64_RS13025	2587233..2587445	+	213	WP_000967408.1	type I toxin-antitoxin system Hok family toxin	Toxin
E4U64_RS13030	2587742..2587909	+	168	WP_000998188.1	hypothetical protein	-
E4U64_RS13035	2587975..2588253	+	279	WP_001304183.1	hypothetical protein	-
E4U64_RS13040	2588255..2589304	+	1050	Protein_2546	DUF968 domain-containing protein	-
E4U64_RS13045	2589317..2589691	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
E4U64_RS13050	2589688..2590509	+	822	WP_000762904.1	antitermination protein	-
E4U64_RS13055	2590736..2590933	+	198	WP_000917735.1	hypothetical protein	-
E4U64_RS13060	2591084..2592142	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2515004..2679267	164263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7958.54 Da
• Isoelectric Point: 9.6297

>T121044 WP_000967408.1 NZ_CP038287:2587233-2587445 [Escherichia coli O157:H7]
MLYTRRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 213 bp

>T121044 NZ_CP038287:2587233-2587445 [Escherichia coli O157:H7]
ATGCTCTACACACGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGCT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT121044 NZ_CP038287:c2587278-2587220 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Antitoxin