Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2404332..2404970 Replicon chromosome
Accession NZ_CP038180
Organism Escherichia coli strain

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E4F32_RS11765 Protein ID WP_000813794.1
Coordinates 2404794..2404970 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E4F32_RS11760 Protein ID WP_001270286.1
Coordinates 2404332..2404748 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F32_RS11740 2399484..2400425 - 942 WP_001251313.1 ABC transporter permease -
E4F32_RS11745 2400426..2401439 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
E4F32_RS11750 2401457..2402602 - 1146 WP_000047421.1 ABC transporter substrate-binding protein -
E4F32_RS11755 2402847..2404253 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
E4F32_RS11760 2404332..2404748 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E4F32_RS11765 2404794..2404970 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E4F32_RS11770 2405192..2405422 + 231 WP_000494244.1 YncJ family protein -
E4F32_RS11775 2405514..2407475 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E4F32_RS11780 2407548..2408084 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
E4F32_RS11785 2408137..2409351 + 1215 WP_071607224.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T120763 WP_000813794.1 NZ_CP038180:c2404970-2404794 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T120763 NZ_CP038180:c2404970-2404794 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT120763 WP_001270286.1 NZ_CP038180:c2404748-2404332 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT120763 NZ_CP038180:c2404748-2404332 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References