Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 161814..162454 Replicon plasmid pAsa5-3432
Accession NZ_CP038103
Organism Aeromonas salmonicida subsp. salmonicida strain SHY16-3432

Toxin (Protein)


Gene name vagD Uniprot ID A0A807Z4U5
Locus tag E3D14_RS23395 Protein ID WP_005320982.1
Coordinates 162041..162454 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A807Z5T0
Locus tag E3D14_RS23390 Protein ID WP_005320985.1
Coordinates 161814..162044 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3D14_RS23375 159479..159841 - 363 WP_005321010.1 hypothetical protein -
E3D14_RS23380 159881..160327 - 447 WP_043145058.1 DNA repair protein RadC -
E3D14_RS23385 161184..161745 + 562 Protein_175 site-specific integrase -
E3D14_RS23390 161814..162044 + 231 WP_005320985.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E3D14_RS23395 162041..162454 + 414 WP_005320982.1 PIN domain-containing protein Toxin
E3D14_RS23400 162529..162894 + 366 WP_043145064.1 hypothetical protein -
E3D14_RS23405 162910..163410 + 501 WP_005320977.1 hypothetical protein -
E3D14_RS23410 163432..163740 + 309 WP_005320974.1 hypothetical protein -
E3D14_RS23415 163846..164055 + 210 Protein_181 helix-turn-helix domain-containing protein -
E3D14_RS23420 164277..165818 + 1542 WP_005322071.1 IS21 family transposase -
E3D14_RS23425 165833..166588 + 756 WP_005322068.1 IS21-like element ISAs29 family helper ATPase IstB -
E3D14_RS23435 167125..167277 + 153 WP_005322083.1 Hok/Gef family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA2 / blaCARB-2 / tet(G) / floR / tet(A) / mcr-3.1 / mcr-3.15 sycH / aopH / ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC / ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP / ascQ / ascR / ascS / ascT / ascU / aopO 1..180043 180043


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.7146

>T120694 WP_005320982.1 NZ_CP038103:162041-162454 [Aeromonas salmonicida subsp. salmonicida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Download         Length: 414 bp

>T120694 NZ_CP038103:162041-162454 [Aeromonas salmonicida subsp. salmonicida]
GTGAAGACGTTTATGCTCGATACCTGCATTTGTTCCTTCATCATGCGCGAAATGCCACAGTCAGTTCTTGAACGGTTGGG
CGCAGAGGTGGCGAGCGGCAATCGGATTGTGATCTCGGCAATCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGA
AGGCTTCTCCGAAGCTGGGGCCAGCCATTGATGAATTTGTTCGTCGTCTCGATGGGATCTTGCCATGGGACGCTGCTACG
GTCGATCAGACGCTCGAAGTGCGCCAGCAGTTAGCAGCCTTGGGCACTCCGATCGGCAATAACGATGCTGCTATTGCCGC
CCACGCCCTGACGGCTGGCTGTGTACTGGTAACCAACAATACCCGCGAGTTTTCCCGAGTGGCGGGGCTGCTCTATGAAG
ACTGGGTTCACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8699.88 Da        Isoelectric Point: 4.7419

>AT120694 WP_005320985.1 NZ_CP038103:161814-162044 [Aeromonas salmonicida subsp. salmonicida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Download         Length: 231 bp

>AT120694 NZ_CP038103:161814-162044 [Aeromonas salmonicida subsp. salmonicida]
ATGCGTACTGTATCAATTTTTAAAAATGGTAAAAATCAGGCGGTGAGACTGCCAAAAGACATGGAGTTTCAGGGGGTTAA
TGACCTTGAGATTATCAAAAATGGCGACACAATCACGTTGCGCCCGGCCCGCCCTGATTGGTTTTCCCTGGCGGAATGCC
AGAAGGTGGATGCAGATTTCTTGCAAGAACGTACCGATGTGATCAGCGATGAAGGACGGTTCGCCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807Z4U5

Antitoxin

Source ID Structure
AlphaFold DB A0A807Z5T0

References