Detailed information of TA system TA120646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelB-YoeB
• Location: 291912..292420
• Replicon: chromosome
• Accession: NZ_CP038017
• Organism: Allofrancisella frigidaquae strain SYSU 10HL1970

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M3HS47
• Locus tag: E3E15_RS01350
• Protein ID: WP_035721097.1
• Coordinates: 291912..292169 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E3E15_RS01355
• Protein ID: WP_172106304.1
• Coordinates: 292166..292420 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3E15_RS01325	287918..288529	-	612	WP_172106300.1	dephospho-CoA kinase	-
E3E15_RS01330	288658..289794	+	1137	WP_172106301.1	SAM-dependent methyltransferase	-
E3E15_RS01335	289804..291180	+	1377	WP_172106302.1	phosphomannomutase/phosphoglucomutase	-
E3E15_RS01340	291391..291573	+	183	WP_172106303.1	hypothetical protein	-
E3E15_RS01345	291571..291797	-	227	Protein_256	transposase	-
E3E15_RS01350	291912..292169	+	258	WP_035721097.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Toxin
E3E15_RS01355	292166..292420	+	255	WP_172106304.1	Txe/YoeB family addiction module toxin	Antitoxin
E3E15_RS01360	292656..292784	-	129	WP_172106305.1	transposase	-
E3E15_RS01365	292813..292987	+	175	Protein_260	IS982 family transposase	-
E3E15_RS01370	293154..294629	+	1476	WP_172106306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9698.88 Da
• Isoelectric Point: 4.6104

>T120646 WP_035721097.1 NZ_CP038017:291912-292169 [Allofrancisella frigidaquae]
MRTLNYTSFRSELASSMDSVVEDHQPIVVTRGSEKKAVVVMSLDDFKSYQETAHLMSSINNYNRLDQSIKELESGISIQK
ELIEE

Nucleotide

• Length: 258 bp

>T120646 NZ_CP038017:291912-292169 [Allofrancisella frigidaquae]
ATGAGAACTTTAAACTATACATCATTCAGAAGTGAACTGGCATCATCTATGGATAGCGTCGTTGAAGACCATCAGCCTAT
TGTTGTTACAAGAGGCTCTGAAAAAAAAGCTGTTGTTGTTATGTCTTTAGATGATTTTAAATCATATCAAGAAACAGCTC
ATTTGATGTCTAGCATAAATAACTATAATAGGCTTGATCAATCAATAAAAGAACTTGAGTCTGGTATTTCAATACAAAAA
GAGCTTATTGAAGAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 10431.89 Da
• Isoelectric Point: 9.7954

>AT120646 WP_172106304.1 NZ_CP038017:292166-292420 [Allofrancisella frigidaquae]
MILSWSTNAWDDYLYWQANDKKKLKRINQLIKDIKRNYFEGLGDPEPLKHSWTGYWSRRIDREHRLVYKIQDNNILIAQC
RYHY

Nucleotide

• Length: 255 bp

>AT120646 NZ_CP038017:292166-292420 [Allofrancisella frigidaquae]
ATGATATTATCTTGGTCAACTAATGCTTGGGATGATTATCTTTATTGGCAAGCAAATGATAAGAAAAAGTTAAAGCGTAT
AAATCAGCTAATTAAAGATATTAAAAGAAATTATTTTGAAGGTTTGGGTGATCCAGAACCACTTAAACATAGTTGGACTG
GATATTGGTCAAGAAGAATTGATAGAGAACATCGCTTAGTTTATAAAATTCAGGATAATAATATTCTTATTGCTCAATGC
AGGTATCACTATTAA

Structures

Toxin

• AlphaFold DB: A0A6M3HS47