Detailed information of TA system TA120632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1526812..1527416
• Replicon: chromosome
• Accession: NZ_CP038001
• Organism: Pseudomonas sp. SXM-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: DCC84_RS07135
• Protein ID: WP_134325879.1
• Coordinates: 1527123..1527416 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1R0GAX7
• Locus tag: DCC84_RS07130
• Protein ID: WP_003212064.1
• Coordinates: 1526812..1527120 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCC84_RS07100	1522322..1522654	-	333	WP_134325876.1	hypothetical protein	-
DCC84_RS07105	1522677..1523087	-	411	WP_003212074.1	type II toxin-antitoxin system HicB family antitoxin	-
DCC84_RS07110	1523111..1523293	-	183	WP_003189411.1	type II toxin-antitoxin system HicA family toxin	-
DCC84_RS07115	1523465..1524373	-	909	WP_087692622.1	LysR family transcriptional regulator	-
DCC84_RS07120	1524469..1525632	+	1164	WP_134325877.1	MFS transporter	-
DCC84_RS07125	1525629..1526819	+	1191	WP_134325878.1	MFS transporter	-
DCC84_RS07130	1526812..1527120	-	309	WP_003212064.1	putative addiction module antidote protein	Antitoxin
DCC84_RS07135	1527123..1527416	-	294	WP_134325879.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DCC84_RS07140	1527578..1528390	-	813	WP_008437810.1	helix-turn-helix transcriptional regulator	-
DCC84_RS07145	1528486..1529376	+	891	WP_102604154.1	SDR family oxidoreductase	-
DCC84_RS07150	1529383..1529651	+	269	Protein_1346	GNAT family N-acetyltransferase	-
DCC84_RS07155	1529701..1530051	-	351	WP_134325880.1	BrnA antitoxin family protein	-
DCC84_RS07160	1530055..1530336	-	282	WP_008437813.1	BrnT family toxin	-
DCC84_RS34330	1530680..1530826	+	147	WP_158244060.1	hypothetical protein	-
DCC84_RS07165	1530912..1531718	+	807	WP_008437814.1	polysaccharide lyase family 7 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10716.36 Da
• Isoelectric Point: 10.8628

>T120632 WP_134325879.1 NZ_CP038001:c1527416-1527123 [Pseudomonas sp. SXM-1]
VNYLVQQTQTFITWHSAILDLRAKIAIARRIDRASAGNLGDIKTVGNGVSEMRVDIGLGYRVYFTQRGRTVIVLLAGGDK
SSQDADIRRAKKLAKEV

Nucleotide

• Length: 294 bp

>T120632 NZ_CP038001:c1527416-1527123 [Pseudomonas sp. SXM-1]
GTGAACTATCTAGTCCAGCAAACGCAGACCTTCATTACCTGGCATTCAGCAATACTTGATTTACGCGCAAAAATCGCCAT
CGCCCGGCGCATCGATCGCGCATCTGCTGGCAATCTGGGCGATATAAAGACGGTAGGCAATGGTGTCTCGGAAATGCGCG
TAGATATCGGTCTGGGTTATCGAGTGTATTTCACGCAGCGAGGAAGAACCGTCATCGTCTTGCTGGCCGGTGGCGACAAA
TCCTCACAAGACGCCGACATCCGGCGAGCAAAAAAACTGGCAAAGGAAGTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10981.49 Da
• Isoelectric Point: 5.0484

>AT120632 WP_003212064.1 NZ_CP038001:c1527120-1526812 [Pseudomonas sp. SXM-1]
MSQTLTTFDMAALLDSDEAISEYLSQVLADGDNEEFLRAVGYVAKARGMARVATDTGMGRESLYKAFAPGAKPRFETVLK
VLHALGIDLQAQPGHVANHSII

Nucleotide

• Length: 309 bp

>AT120632 NZ_CP038001:c1527120-1526812 [Pseudomonas sp. SXM-1]
ATGAGCCAAACACTGACGACATTTGATATGGCGGCACTGCTCGACAGTGATGAAGCCATCAGCGAATACCTTTCTCAAGT
TCTGGCCGACGGCGATAATGAGGAGTTCCTCAGGGCCGTCGGGTACGTGGCCAAAGCTCGCGGGATGGCGCGGGTCGCGA
CCGATACCGGCATGGGCCGTGAGAGTCTGTACAAGGCATTTGCCCCTGGAGCAAAGCCCCGCTTCGAAACGGTACTCAAG
GTGCTTCACGCACTGGGTATCGATCTGCAAGCACAGCCGGGGCATGTCGCCAATCATTCGATTATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1R0GAX7