Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 5531917..5532712 | Replicon | chromosome |
| Accession | NZ_CP037943 | ||
| Organism | Escherichia coli strain CFSAN027343 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | EKI57_RS28605 | Protein ID | WP_000854914.1 |
| Coordinates | 5532338..5532712 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A7U9LM11 |
| Locus tag | EKI57_RS28600 | Protein ID | WP_001280953.1 |
| Coordinates | 5531917..5532291 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKI57_RS28565 | 5527272..5528177 | + | 906 | WP_000203541.1 | chemotaxis protein | - |
| EKI57_RS28570 | 5528174..5529244 | + | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| EKI57_RS28580 | 5529584..5530402 | + | 819 | WP_001234682.1 | DUF945 domain-containing protein | - |
| EKI57_RS28585 | 5530493..5530978 | + | 486 | WP_000214398.1 | antirestriction protein | - |
| EKI57_RS28590 | 5530994..5531470 | + | 477 | WP_001186738.1 | RadC family protein | - |
| EKI57_RS28595 | 5531533..5531754 | + | 222 | WP_000692323.1 | DUF987 domain-containing protein | - |
| EKI57_RS28600 | 5531917..5532291 | + | 375 | WP_001280953.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| EKI57_RS28605 | 5532338..5532712 | + | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| EKI57_RS28610 | 5532709..5533200 | + | 492 | WP_000976857.1 | hypothetical protein | - |
| EKI57_RS28615 | 5533212..5533409 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| EKI57_RS28620 | 5533494..5534336 | + | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| EKI57_RS28630 | 5534807..5535745 | + | 939 | WP_000351317.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| EKI57_RS28635 | 5535800..5536537 | + | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| EKI57_RS28640 | 5536561..5537115 | + | 555 | WP_001001902.1 | molecular chaperone YcdY | - |
| EKI57_RS28645 | 5537217..5537708 | + | 492 | WP_001297187.1 | DUF1097 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | csgG / csgF / csgE / csgD / csgB / csgA / csgC | 5520176..5543273 | 23097 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T120496 WP_000854914.1 NZ_CP037943:5532338-5532712 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T120496 NZ_CP037943:5532338-5532712 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13734.56 Da Isoelectric Point: 6.6249
>AT120496 WP_001280953.1 NZ_CP037943:5531917-5532291 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS
Download Length: 375 bp
>AT120496 NZ_CP037943:5531917-5532291 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LM11 |