Detailed information of TA system TA120123

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2563452..2563654
• Replicon: chromosome
• Accession: NZ_CP037827
• Organism: Clostridioides difficile strain Cd10

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: E1H33_RS12100
• Protein ID: WP_004454589.1
• Coordinates: 2563502..2563654 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2563452..2563581 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H33_RS12065 (2558562)	2558562..2558879	+	318	WP_009897465.1	hypothetical protein	-
E1H33_RS12070 (2559015)	2559015..2559479	+	465	WP_004454576.1	hypothetical protein	-
E1H33_RS19730 (2559508)	2559508..2559633	-	126	Protein_2313	hypothetical protein	-
E1H33_RS12075 (2559808)	2559808..2559969	-	162	WP_004454578.1	hypothetical protein	-
E1H33_RS12080 (2560021)	2560021..2560473	-	453	WP_021359435.1	hypothetical protein	-
E1H33_RS12085 (2560470)	2560470..2560835	-	366	WP_016729316.1	Bro-N domain-containing protein	-
E1H33_RS12090 (2560955)	2560955..2561107	-	153	WP_009897477.1	hypothetical protein	-
E1H33_RS12095 (2562424)	2562424..2563233	-	810	WP_018112791.1	hypothetical protein	-
- (2563452)	2563452..2563581	+	130	NuclAT_3	-	Antitoxin
- (2563452)	2563452..2563581	+	130	NuclAT_3	-	Antitoxin
- (2563452)	2563452..2563581	+	130	NuclAT_5	-	Antitoxin
E1H33_RS12100 (2563502)	2563502..2563654	-	153	WP_004454589.1	hypothetical protein	Toxin
E1H33_RS12105 (2563928)	2563928..2564251	-	324	WP_009897482.1	DUF6054 family protein	-
E1H33_RS12110 (2564287)	2564287..2564541	-	255	WP_004454592.1	hypothetical protein	-
E1H33_RS12115 (2564973)	2564973..2565491	-	519	Protein_2322	transposase	-
E1H33_RS12120 (2565781)	2565781..2566155	-	375	Protein_2323	BlaI/MecI/CopY family transcriptional regulator	-
E1H33_RS12125 (2566260)	2566260..2566634	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
E1H33_RS12130 (2567438)	2567438..2567926	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E1H33_RS12135 (2568311)	2568311..2568485	+	175	Protein_2326	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T120123 WP_004454589.1 NZ_CP037827:c2563654-2563502 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T120123 NZ_CP037827:c2563654-2563502 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT120123 NZ_CP037827:2563452-2563581 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin