Detailed information of TA system TA120085

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1782454..1782668
• Replicon: chromosome
• Accession: NZ_CP037818
• Organism: Clostridioides difficile strain Cd14

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: E1H37_RS08720
• Protein ID: WP_003429855.1
• Coordinates: 1782454..1782615 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1782535..1782668 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1H37_RS08685 (1777641)	1777641..1777931	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
E1H37_RS08690 (1778041)	1778041..1778847	+	807	WP_021358994.1	hypothetical protein	-
E1H37_RS08695 (1779196)	1779196..1779633	+	438	WP_021362375.1	hypothetical protein	-
E1H37_RS08700 (1779626)	1779626..1779802	+	177	WP_021362376.1	hypothetical protein	-
E1H37_RS08705 (1779804)	1779804..1781114	+	1311	WP_021368245.1	phage tail sheath family protein	-
E1H37_RS08710 (1781131)	1781131..1781601	+	471	WP_003429851.1	phage tail tube protein	-
E1H37_RS08715 (1781673)	1781673..1782113	+	441	WP_003429853.1	phage portal protein	-
E1H37_RS08720 (1782454)	1782454..1782615	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1782535)	1782535..1782668	-	134	NuclAT_2	-	Antitoxin
- (1782535)	1782535..1782668	-	134	NuclAT_2	-	Antitoxin
- (1782535)	1782535..1782668	-	134	NuclAT_3	-	Antitoxin
- (1782535)	1782535..1782668	-	134	NuclAT_5	-	Antitoxin
- (1783013)	1783013..1783118	-	106	NuclAT_5	-	-
- (1783013)	1783013..1783118	-	106	NuclAT_5	-	-
- (1783013)	1783013..1783118	-	106	NuclAT_8	-	-
- (1783013)	1783013..1783118	-	106	NuclAT_9	-	-
E1H37_RS08725 (1783940)	1783940..1784113	+	174	WP_018112722.1	hypothetical protein	-
E1H37_RS08730 (1784237)	1784237..1785064	+	828	WP_021368246.1	hypothetical protein	-
E1H37_RS08735 (1785127)	1785127..1785303	+	177	WP_009894378.1	hypothetical protein	-
E1H37_RS08740 (1785716)	1785716..1785919	-	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
E1H37_RS19735 (1786163)	1786163..1786966	+	804	WP_021377655.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T120085 WP_003429855.1 NZ_CP037818:1782454-1782615 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T120085 NZ_CP037818:1782454-1782615 [Clostridioides difficile]
ATGAATAACTTTTTACTTAATGTAATAGCTGGCGTTATTGCTAGTTTAATATTTTGCTTAATTTGTAAAGTATTTCTAAA
AGTAAAAAGCCACTCAACTCGTGGCAAGAGTAAAAGTGGCTGGGAATTTGATTTTAAAATCAAGTTCCATAAGTTCAAAT
AG

Antitoxin

Nucleotide

• Length: 134 bp

>AT120085 NZ_CP037818:c1782668-1782535 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin