Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3303257..3303848 Replicon chromosome
Accession NZ_CP036514
Organism Hafnia alvei strain PCM_1220

Toxin (Protein)


Gene name Hha Uniprot ID A0A097R540
Locus tag EYZ02_RS15485 Protein ID WP_025799954.1
Coordinates 3303645..3303848 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A097R537
Locus tag EYZ02_RS15480 Protein ID WP_025799952.1
Coordinates 3303257..3303625 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYZ02_RS15465 3299019..3302171 + 3153 WP_025799949.1 multidrug efflux RND transporter permease subunit -
EYZ02_RS15470 3302332..3302604 + 273 WP_025799950.1 type B 50S ribosomal protein L31 -
EYZ02_RS15475 3302604..3302744 + 141 WP_004091741.1 type B 50S ribosomal protein L36 -
EYZ02_RS15480 3303257..3303625 + 369 WP_025799952.1 Hha toxicity modulator TomB Antitoxin
EYZ02_RS15485 3303645..3303848 + 204 WP_025799954.1 hemolysin expression modulator Hha Toxin
EYZ02_RS15490 3304049..3304468 - 420 WP_025799956.1 DUF1311 domain-containing protein -
EYZ02_RS15500 3304954..3305292 + 339 WP_115348070.1 MGMT family protein -
EYZ02_RS15505 3305340..3305858 - 519 WP_025799960.1 YbaY family lipoprotein -
EYZ02_RS15510 3306151..3307011 + 861 WP_131391928.1 acyl-CoA thioesterase II -
EYZ02_RS15515 3307092..3308385 - 1294 Protein_2987 ammonium transporter AmtB -
EYZ02_RS15520 3308416..3308754 - 339 WP_004091732.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8133.40 Da        Isoelectric Point: 5.8423

>T119922 WP_025799954.1 NZ_CP036514:3303645-3303848 [Hafnia alvei]
MTKIDYLMRLRKCTTLETLERVIEKNKYELSDDELETFYSAADHRLAELTMNKLYDKIPAEVWKYVR

Download         Length: 204 bp

>T119922 NZ_CP036514:3303645-3303848 [Hafnia alvei]
ATGACTAAAATTGATTACCTTATGCGCTTACGCAAGTGTACAACGTTAGAAACTTTAGAGCGTGTAATTGAAAAAAACAA
ATACGAGCTCTCCGACGACGAGCTAGAAACTTTCTACTCAGCCGCCGACCATCGTTTGGCCGAACTTACCATGAACAAAC
TGTATGATAAGATCCCTGCTGAAGTTTGGAAGTATGTTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14201.03 Da        Isoelectric Point: 4.7386

>AT119922 WP_025799952.1 NZ_CP036514:3303257-3303625 [Hafnia alvei]
MDEYTSYQHDITELKYLCNMLYNQGMEVLSDSHHGWVNDPTAVANLQLNELNEHIATFGLTFKIKYPKSSELTEILDEYL
DETYSLFSNYSINEPELKKWLKVKGRILRYLAGEKPASALNF

Download         Length: 369 bp

>AT119922 NZ_CP036514:3303257-3303625 [Hafnia alvei]
ATGGATGAGTACACGTCTTATCAGCACGATATAACTGAACTGAAATACCTTTGTAATATGCTGTATAATCAGGGAATGGA
AGTTCTGAGTGATAGCCATCATGGCTGGGTTAATGACCCAACTGCTGTAGCTAATCTGCAACTCAATGAATTAAACGAAC
ATATCGCAACGTTCGGTTTAACGTTCAAGATTAAGTATCCAAAGAGTTCAGAATTAACTGAAATATTAGATGAGTATCTT
GACGAAACTTACAGTCTGTTTAGTAATTACAGCATAAACGAACCAGAACTAAAAAAATGGCTTAAAGTAAAAGGCCGTAT
TTTGCGTTATTTAGCGGGTGAAAAACCAGCATCCGCGTTGAACTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097R540

Antitoxin

Source ID Structure
AlphaFold DB A0A097R537

References