Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 35664..36187 Replicon plasmid unnamed1
Accession NZ_CP036446
Organism Klebsiella pneumoniae strain KPNIH45

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag C3377_RS00220 Protein ID WP_015632464.1
Coordinates 35664..35969 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag C3377_RS00225 Protein ID WP_015632465.1
Coordinates 35969..36187 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3377_RS00175 30694..31122 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
C3377_RS00180 31115..32095 - 981 WP_015632457.1 partitioning protein -
C3377_RS00185 32456..32638 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
C3377_RS00190 32664..33107 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
C3377_RS00195 33313..33435 - 123 Protein_37 transcriptional regulator -
C3377_RS00200 33449..33676 - 228 WP_016162072.1 hypothetical protein -
C3377_RS00205 34106..34363 + 258 WP_016162071.1 helix-turn-helix domain-containing protein -
C3377_RS00210 34567..35133 - 567 WP_015632462.1 hypothetical protein -
C3377_RS00215 35175..35519 - 345 WP_015632463.1 hypothetical protein -
C3377_RS00220 35664..35969 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
C3377_RS00225 35969..36187 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C3377_RS00230 36319..36804 + 486 Protein_44 IS5 family transposase -
C3377_RS00235 36968..37399 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
C3377_RS00240 37399..38670 + 1272 WP_016162068.1 Y-family DNA polymerase -
C3377_RS00245 39082..39957 + 876 WP_004098982.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia - 1..96395 96395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T119820 WP_015632464.1 NZ_CP036446:c35969-35664 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T119820 NZ_CP036446:c35969-35664 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT119820 WP_015632465.1 NZ_CP036446:c36187-35969 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT119820 NZ_CP036446:c36187-35969 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References