Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 174588..175324 Replicon plasmid pIncFIBK
Accession NZ_CP036337
Organism Klebsiella pneumoniae strain BP327

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag EYB55_RS29225 Protein ID WP_003026803.1
Coordinates 174842..175324 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag EYB55_RS29220 Protein ID WP_003026799.1
Coordinates 174588..174854 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYB55_RS29175 170656..171018 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
EYB55_RS29180 171068..171418 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
EYB55_RS29185 171776..172045 + 270 WP_004152102.1 hypothetical protein -
EYB55_RS29190 172033..172608 + 576 WP_004152103.1 hypothetical protein -
EYB55_RS29195 172639..173131 + 493 Protein_183 DNA-binding protein -
EYB55_RS29200 173176..173544 + 369 WP_004152105.1 hypothetical protein -
EYB55_RS29205 173577..173780 + 204 WP_004152106.1 hemolysin expression modulator Hha -
EYB55_RS29210 173829..174082 + 254 Protein_186 hypothetical protein -
EYB55_RS29215 174158..174412 + 255 WP_004152108.1 hypothetical protein -
EYB55_RS29220 174588..174854 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
EYB55_RS29225 174842..175324 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
EYB55_RS29230 175525..176926 + 1402 Protein_190 ISNCY-like element ISKpn21 family transposase -
EYB55_RS29235 176955..177587 - 633 WP_001567369.1 hypothetical protein -
EYB55_RS29240 177817..179163 + 1347 WP_020314316.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / aph(3')-Ia / tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 - 1..225575 225575


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T119607 WP_003026803.1 NZ_CP036337:174842-175324 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T119607 NZ_CP036337:174842-175324 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT119607 WP_003026799.1 NZ_CP036337:174588-174854 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT119607 NZ_CP036337:174588-174854 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References