Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2369285..2369939 Replicon chromosome
Accession NZ_CP036202
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag D9K49_RS11440 Protein ID WP_000244772.1
Coordinates 2369532..2369939 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag D9K49_RS11435 Protein ID WP_000354046.1
Coordinates 2369285..2369551 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS11410 2364454..2365197 + 744 WP_000951964.1 SDR family oxidoreductase -
D9K49_RS11415 2365254..2366687 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
D9K49_RS11420 2366732..2367043 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
D9K49_RS11425 2367207..2367866 + 660 WP_000250274.1 hemolysin III family protein -
D9K49_RS11430 2368062..2369042 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
D9K49_RS11435 2369285..2369551 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
D9K49_RS11440 2369532..2369939 + 408 WP_000244772.1 protein YgfX Toxin
D9K49_RS11445 2369979..2370500 - 522 WP_001055874.1 flavodoxin FldB -
D9K49_RS11450 2370612..2371508 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
D9K49_RS11455 2371533..2372243 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
D9K49_RS11460 2372249..2373982 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T119351 WP_000244772.1 NZ_CP036202:2369532-2369939 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T119351 NZ_CP036202:2369532-2369939 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT119351 WP_000354046.1 NZ_CP036202:2369285-2369551 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT119351 NZ_CP036202:2369285-2369551 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References