Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245529..246171 Replicon chromosome
Accession NZ_CP036117
Organism Bacillus mycoides strain JAS481

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW43_RS02065 Protein ID WP_000635965.1
Coordinates 245821..246171 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW43_RS02060 Protein ID WP_000004570.1
Coordinates 245529..245816 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW43_RS02035 (EXW43_02130) 240694..241656 + 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -
EXW43_RS02040 (EXW43_02135) 241823..242371 - 549 WP_080684121.1 rhomboid family intramembrane serine protease -
EXW43_RS02045 (EXW43_02140) 242464..242823 + 360 WP_002009969.1 holo-ACP synthase -
EXW43_RS02050 (EXW43_02145) 242980..243930 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
EXW43_RS02055 (EXW43_02150) 244048..245217 + 1170 WP_002124821.1 alanine racemase -
EXW43_RS02060 (EXW43_02155) 245529..245816 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW43_RS02065 (EXW43_02160) 245821..246171 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW43_RS02070 (EXW43_02165) 246240..248408 + 2169 WP_002124824.1 Tex family protein -
EXW43_RS02075 (EXW43_02170) 248466..248582 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW43_RS02080 (EXW43_02175) 248793..249248 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T119218 WP_000635965.1 NZ_CP036117:245821-246171 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T119218 NZ_CP036117:245821-246171 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT119218 WP_000004570.1 NZ_CP036117:245529-245816 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT119218 NZ_CP036117:245529-245816 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References