Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2891991..2892469 Replicon chromosome
Accession NZ_CP035860
Organism Escherichia coli strain TD225-C4

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A7D8WGY6
Locus tag EXA00_RS14305 Protein ID WP_000936798.1
Coordinates 2892182..2892469 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID D8A5B4
Locus tag EXA00_RS14300 Protein ID WP_000100899.1
Coordinates 2891991..2892182 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXA00_RS26250 (2887037) 2887037..2887159 - 123 WP_077763265.1 DUF551 domain-containing protein -
EXA00_RS26255 (2887189) 2887189..2887617 - 429 Protein_2795 DUF551 domain-containing protein -
EXA00_RS14260 (2887855) 2887855..2888310 - 456 WP_032200358.1 ead/Ea22-like family protein -
EXA00_RS14265 (2888297) 2888297..2888602 - 306 WP_032200359.1 DUF4406 domain-containing protein -
EXA00_RS14270 (2888599) 2888599..2889015 - 417 WP_001409082.1 DUF977 family protein -
EXA00_RS14275 (2889030) 2889030..2889776 - 747 WP_216334050.1 ATP-binding protein -
EXA00_RS14280 (2889783) 2889783..2890748 - 966 WP_216334051.1 hypothetical protein -
EXA00_RS14285 (2890771) 2890771..2891196 - 426 WP_032207470.1 toxin YdaT family protein -
EXA00_RS14290 (2891180) 2891180..2891452 - 273 WP_000887447.1 YdaS family helix-turn-helix protein -
EXA00_RS14295 (2891562) 2891562..2891963 + 402 WP_001329851.1 helix-turn-helix domain-containing protein -
EXA00_RS14300 (2891991) 2891991..2892182 + 192 WP_000100899.1 hypothetical protein Antitoxin
EXA00_RS14305 (2892182) 2892182..2892469 + 288 WP_000936798.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EXA00_RS14310 (2892742) 2892742..2892897 + 156 WP_216334053.1 DUF1391 family protein -
EXA00_RS14315 (2893057) 2893057..2893275 + 219 WP_001171921.1 protein YdfC -
EXA00_RS14320 (2893298) 2893298..2893903 + 606 WP_216334055.1 hypothetical protein -
EXA00_RS14325 (2893881) 2893881..2894072 - 192 WP_052931385.1 hypothetical protein -
EXA00_RS14330 (2894638) 2894638..2894826 + 189 WP_000449198.1 cell division inhibition protein DicB -
EXA00_RS14335 (2894823) 2894823..2895026 + 204 WP_001532593.1 DUF1482 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2852476..2904119 51643


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10899.83 Da        Isoelectric Point: 10.1360

>T118870 WP_000936798.1 NZ_CP035860:2892182-2892469 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENIPAARRLKIRIETSVLALSEHPYLYPPSDRVSGLREIVVHPNYIVLYRVAASSIE
IANIVHARRQFPFPI

Download         Length: 288 bp

>T118870 NZ_CP035860:2892182-2892469 [Escherichia coli]
ATGTTACCCATTTTGTGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGGATTGAAACGTCTGTTTTAGCCCTCTCTGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAGCTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7035.80 Da        Isoelectric Point: 4.0270

>AT118870 WP_000100899.1 NZ_CP035860:2891991-2892182 [Escherichia coli]
MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDEVMAEMENLIAQIAVTNRSE

Download         Length: 192 bp

>AT118870 NZ_CP035860:2891991-2892182 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTACGCGC
TAAAGTGGCGTCAAGCCTTGCTGACCCTCGTCCCTCAATTCCACATGATGAGGTAATGGCTGAAATGGAGAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D8WGY6

Antitoxin

Source ID Structure
AlphaFold DB A0A161RC07

References